Imbeba Umurizo ADN Mini Kit Genomic ADN Ikuramo Ibikoresho biva murizo
Ibisobanuro:
Igikoresho cyihuta cyane kwisi gukuramo ADN ya genomic ADN, ishobora gukuramo ADN nziza cyane mumirizo yimbeba mugihe cyisaha 1.
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
Ibisobanuro
Bitewe n'imiterere n'imiterere y'umurizo wimbeba ubwayo, gukuramo ADN umurizo wimbeba ADN yamye ihangayikishije abashakashatsi: kimwe nuko igihe cyo gutunganya umurizo wimbeba ari kirekire kandi ADN ya genomic ikangirika byoroshye, indi igoye kubyitwaramo kandi ubuziranenge bwa ADN yakuweho ni buke.
Iki gikoresho gikoresha ADN-Inkingi yonyine ishobora guhuza ADN, protease nshya ya Foregene Protease hamwe na sisitemu idasanzwe ya buffer, ishobora gusya ingero z’umurizo w’imbeba mu minota 45, bityo bikagabanya iyangirika rya ADN ya genomique kandi bigabanya igihe cyo gutunganya icyitegererezo.Igikoresho kirashobora gukuramo 10-20μg ya ADN yujuje ubuziranenge, ifite isuku nyinshi ya ADN kuva 0.5-1cm umurizo wimbeba cyangwa imbeba zikuze muri 60-90min.
Ibikoresho bya ADN byonyine bya silika gel matrix bikoreshwa mukuzunguruka ni ibintu bishya bidasanzwe byikigo, bishobora gukora neza kandi byumwihariko kwamamaza ADN.Ubushobozi ntarengwa bwa adsorption kuri ADN ni 80μg.Sisitemu idasanzwe na sisitemu yo gukuraho irashobora gukuraho cyane gukuraho RNA, poroteyine zanduye, ion hamwe n’ibindi binyabuzima mu ngirabuzimafatizo.Igice cya ADN cyakuweho ni kinini, gifite isuku nyinshi, gihamye kandi cyizewe mu bwiza, kandi ubunini bwa ADN buhagaze neza kuri 23kb.
Igikoresho kirashobora kandi gukoreshwa mugukuramo izindi nyama zimbeba hanze umurizo wimbeba, harimo ugutwi kwimbeba, imitsi yimbeba, umwijima wimbeba nizindi ngingo, kugirango igikoresho kimwe gishobora gukoreshwa mubikorwa byinshi kandi gihuza ibyifuzo byabakoresha mubushakashatsi butandukanye.
Ibisobanuro
50 Imyiteguro, 100 Itegura, 250 Itegura
Ibigize ibikoresho
| Buffer TL1 |
| Buffer TL2 * |
| Buffer PW * |
| Buffer WB |
| Buffer EB |
| Foregene Protease Yongeyeho |
| ADN-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Nta kwanduza RNase: Inkingi ya ADN-Yonyine yatanzwe nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utiriwe wongera RNase mugihe cyigeragezo, ukirinda laboratoire kwanduzwa na RNase idasanzwe.
-Umuvuduko wihuse: Foregene Protease Plus ifite ibikorwa byinshi kuruta protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 40.
-Ibyoroshye: Centrifugation ikorwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
-Umutekano: Nta gukuramo reagent ikenewe.
-Ubuziranenge bwo hejuru: ADN yakuwe muri ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
Porogaramu
Birakwiriye gukuramo no kweza ADN genomic mumirizo mishya cyangwa ikonje umurizo munini nimbeba (nayo ikoreshwa mugusukura imbeba cyangwa imbeba nimbeba na ADN genomic ADN).
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
-Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C);niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri 2-8 ° C.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
-Foregene Protease Plus igisubizo gifite formula idasanzwe, ikora iyo ibitswe mubushyuhe bwicyumba igihe kinini (amezi 3);bibitswe kuri 4 ° C, ibikorwa no gutuza bizaba byiza, bityo birasabwa kubibika kuri 4 ° C, ibuka kutabika kuri -20 ° C.
