RT Easyᵀᴹ II Master Premix kumurongo wambere wa cDNA synthesis kumwanya nyawo PCR
Ibisobanuro:
- Sisitemu nziza yo kwandukura sisitemu, bifata iminota 20 gusa kugirango urangize synthesis yumurongo wambere cDNA.
- Sisitemu yo hejuru-yimikorere ya sisitemu yo kwandukura, pg-urwego rwicyitegererezo na rwo rushobora kubona cDNA nziza.
- Sisitemu yo kwandukura sisitemu ifite ubushyuhe buhanitse, ubushyuhe bwa reaction ya sisitemu irashobora kuba hejuru ya 50 and, kandi ifite imikorere myiza yo kwandukura.
- Ndetse na RNA yanduye (inzoga zigera kuri 60%, umunyu wa guanidine kugeza kuri 750mM) urashobora gukorerwa reaction ya reaction.
- 2 × RT CYANGWA-ByoroshyeTM Kuvanga byongeweho uburyo bwiza bwo guhindura inyandiko (Primer Primer, Oligo (dT)18 Primer).
Ibisobanuro
Foregene's 2 × RT CYANGWA-ByoroshyeTMKuvanga birashobora gushushanya umurongo wa mbere wa cDNA ku bushyuhe buri hejuru ya 50 ° C, ibyo bikaba bifasha guhindura reaction ya reaction ya reaction ya kabiri ya RNA inyandikorugero.
RNA yasukuwe muri laboratoire ikubiyemo inzoga n’ibisigazwa by’umunyu wa guanidine, bifite imbaraga zikomeye zo kubuza kwandukura inyandiko zinyuranye, bikavamo ingaruka zidashimishije zinyuranye cyangwa uburyo bwo kwandukura buke.Foregene 2 × RT Ivanga ryerekana kwihanganira cyane inzoga n'umunyu wa guanidine.Kwihanganira inzoga nyinshi mu ngero za RNA ni 60%, naho kwihanganira umunyu wa guanidine ni 750mM.Ndetse RNA yanduye irashobora gukoreshwa muburyo bwo kwandukura inyandiko hamwe na Foregene 2 × RT CYANGWA-ByoroshyeTMKuvanga.
2 × RT CYANGWA-ByoroshyeTMKuvanga ni reaction yihuta yo kwandukura reagent idasanzwe yakozwe kubwigihe nyacyo PCR.Sisitemu ifite uburyo bwiza bwo guhindura inyandiko kandi ntabwo ikeneye kongeramo primers, kandi irashobora gukora reaction nziza yo kwandukura ku gipimo gito cya RNA.Sisitemu idasanzwe ya reaction ituma reaction ya RT yoroshye, byihuse kandi neza.Igice cya mbere cDNA synthesis irashobora kurangira muminota 20.Ibicuruzwa birashobora gukoreshwa bifatanije na Foregene ya fluorescent yibicuruzwa byuzuye Igihe nyacyo PCR ByoroshyeTMkubona ibisubizo byujuje ubuziranenge ibisubizo.
Ibisobanuro
| RT BiroroshyeTMII Master Premix kumurongo wambere-cDNA synthesis kumwanya nyawo PCR | |||
| Ibigize ibikoresho | RT-01021 | RT-01022 | RT-01023 |
| 50 T (sisitemu ya 10μl) | 200 T (sisitemu ya 10μl) | 800 T (sisitemu ya 10μl) | |
| 2 × RT CYANGWA-ByoroshyeTMKuvanga | 0.25ml | 1ml | 1ml × 4 |
| Ubusa ddH2O | 1.7ml | 1.7ml | 1.7ml × 3 |
| Igitabo gikubiyemo amabwiriza | Igice 1 | Igice 1 | Igice 1 |
Ibiranga ibyiza
- Sisitemu nziza yo kwandukura sisitemu, bifata iminota 20 gusa kugirango urangize synthesis yumurongo wambere cDNA.
- Sisitemu yo hejuru-yimikorere ya sisitemu yo kwandukura, pg-urwego rwicyitegererezo na rwo rushobora kubona cDNA nziza.
- Sisitemu yo kwandukura sisitemu ifite ubushyuhe buhanitse, ubushyuhe bwa reaction ya sisitemu irashobora kuba hejuru ya 50 and, kandi ifite imikorere myiza yo kwandukura.
- Ndetse na RNA yanduye (inzoga zigera kuri 60%, umunyu wa guanidine kugeza kuri 750mM) urashobora gukorerwa reaction ya reaction.
- 2 × RT CYANGWA-ByoroshyeTMKuvanga byongeweho uburyo bwiza bwo guhindura inyandiko (Primer Primer, Oligo (dT)18Primer).
Porogaramu
- Byakoreshejwe muburyo butaziguye PCR kugirango isesengure mu buryo bwuzuye imvugo ya gene
- Irashobora gusesengura byihuse kandi neza umubare muto wa RNA nka virusi ya RNA
- Hindura inyandiko mvugo ya RNA ifite ibintu byinshi bya GC cyangwa urwego rwisumbuyeho
Urujya n'uruza rw'akazi
Ububiko nubuzima bwiza
1. Imiterere yo gutwara abantu
Inzira yose yubushyuhe bwo hasi yubushyuhe bwo gutwara, kugirango umenye neza ko ibikoresho biri muri <4 ° C.
2. Ububiko
RT BiroroshyeTMII ibitswe kuri -20 ° C.Bika ibicuruzwa muri firigo ihoraho yubushyuhe kuri -20 ° C ako kanya ukimara kuyakira.Niba uburyo bwo kubika bukwiye, ibicuruzwa ntibishobora gutesha agaciro imikorere iyo ari yo yose mugihe cyumwaka 1.
