Imbuto y'ibihingwa (Kinini) Direct PCR Kit II-UNG (idafite ibikoresho by'icyitegererezo)
Ibisobanuro
Iki gicuruzwa gikoresha uburyo bwihariye bwa lysis reaction, bushobora kurekura byihuse ADN ya genomique yintangarugero yimbuto yibihingwa hamwe na polysaccharide nkeya hamwe na polifenolike (nka: umuceri, ingano, itabi, ibigori, soya, nibindi) kugirango ikoreshwe mubitekerezo bya PCR, kubwibyo birakwiriye cyane cyane kwipimisha nini nini.Kugirango uhuze ibikenewe mu bizamini bitandukanye byo gutahura, iki gikoresho kirashobora gukoresha ubwoko butandukanye bwintangarugero (nkimbuto zose, gukata uduce duto, cyangwa ingero zubutaka bwimbuto nyinshi) mubushakashatsi.Muri byo, kuburugero rwimbuto rugikeneye kumera, urashobora guhitamo guca uduce duto duto (1-5mg) usibye urusoro rwimbuto kugirango ugerageze;kubushakashatsi bugomba gukopororwa mumubare munini wintangarugero, urashobora guhitamo kuvanga ingero nyinshi hanyuma ukazihuza.Nyuma yo gusya mubice bifite diameter ya 0.5mm, ubushakashatsi burashobora gukorwa (byibuze 0.1% byurugero rwicyitegererezo birashobora kuboneka).
Ibisobanuro
50T, 200T, 500T, 2000T
Ibigize ibikoresho
Imbuto y'ibihingwa Direct PCR Kit II-UNG Imbuto y'Ibihingwa (Kinini) Direct PCR Kit-UNG | |||||
Ibikoresho | TP-0314T | TP-03141 | TP-03142 | TP-03143 | |
50 T | 200T | 500T | 2000T | ||
Igice I. | Buffer SP1 | 30ml | 120ml | 100ml × 3 | 400ml × 3 |
Buffer SP2 | 3ml | 10ml | 25ml | 100ml | |
6 × ADN Yipakurura Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml × 4 | |
Igice cya II | 2 × Imbuto PCR byoroshyeTMKuvanga (UNG) | 500μl | 1ml × 2 | 1.7ml × 3 | 1.7ml × 12 |
Igitabo | 1 | 1 | 1 | 1 |
Ibiranga ibyiza
- Ntibikenewe koza ADN bitwara igihe kandi bihenze.
- Icyitegererezo gisabwa ni gito, fata imbuto imwe.
- Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
- Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
- Kurwanya umwanda PCR sisitemu 2 × Imbuto PCR ByoroshyeTMKuvanga (UNG) birashobora gukuraho neza umwanda uterwa nibicuruzwa bya PCR kandi ukemeza umwihariko nukuri kwa amplification.
Urupapuro rw'akazi
Imiterere yo kubika
1.Gutwara abantu
Inzira yose yubushyuhe bwo hasi yubushyuhe bwo gutwara, kugirango tumenye neza ko igice cya II kiri muri <4 ℃.
Ububiko
Igice cya I cy'iki gikoresho kigomba kubikwa ku bushyuhe bw'icyumba cyangwa 2-8 ° C.
Igice cya II kigomba kubikwa kuri -20 ° C.