CCND1 / IGH Amabara abiri Amabara abiri yo guhuza
Ibisobanuro:
Ukurikije ihame rya ADN yuzuzanya, ubushakashatsi bwa CCND1 orange-umutuku na IGH icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.
Ibisobanuro
Ukurikije ihame rya ADN yuzuzanya, ubushakashatsi bwa CCND1 orange-umutuku na IGH icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.
Ibigize ibikoresho
| Ibigize | Ibisobanuro | ||
| 5Ibizamini | 10Ibizamini | 20Ibizamini | |
| CCND1 / IGHfusion Probe | 50μl | 100μl | 200μl |
Porogaramu
Iki gikoresho gikoreshwa kugirango hamenyekane neza imiterere idasanzwe ya gene ya CCND1 / IGH mu byitegererezo bya cytologiya cyangwa ingero za paraffine zashyizwe muri vitro.
Kugaragaza ibisabwa
-Tissue ingero
Ingero za paraffin zashyizwemo ingero zashyizwe muri 10% itagira aho ibogamiye, kandi uburebure bwibice bwari hagati ya 3 na 5 µm.
-Akagari ingero
Kubijyanye n'amagufwa cyangwa amaraso ya peripheri, icyitegererezo gishya kidakwiye kubikwa kuri 4 ° C mugihe kitarenze amasaha 24, kandi guhagarika ingirabuzimafatizo bigomba kubikwa kuri -20 ° C.
Ububiko hamwe nubuzima bwa Shelf
Bibitswe kuri -20 ℃ ± 5 ℃ mu mwijima, byemewe amezi 12.Ubwikorezi buri munsi ya 8 ℃.
