ETV6 Ibice bibiri Kumena Ibice Bitandukanye
Ibisobanuro:
Dukurikije ihame fatizo ryuzuzanya rya ADN, iperereza rya ETV6 orange-umutuku na ETV6 icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.
Ibisobanuro
Dukurikije ihame fatizo ryuzuzanya rya ADN, iperereza rya ETV6 orange-umutuku na ETV6 icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.
Ibigize ibikoresho
| Ibigize | Ibisobanuro | ||
| Ibizamini 5 | Ibizamini 10 | Ibizamini 20 | |
| ETV6 Ibice bibiri Kumena Ibice Bitandukanye | 50μl | 100μl | 200μl |
Porogaramu
Iki gikoresho gikoreshwa kugirango hamenyekane ubuziranenge bwa ETV6 human 12p13） gene mu cyitegererezo cya cytologiya cyangwa ingero za paraffine zashyizwe muri vitro.
Kugaragaza ibisabwa
-Tissue ingero
Ingero za paraffin zashyizwemo ingero zashyizwe muri 10% itagira aho ibogamiye, kandi uburebure bwibice bwari hagati ya 3 na 5 µm.
-Akagari ingero
Ingero nshya z'amagufwa n'amaraso ya periferiya zabitswe kuri 4 ℃ mu gihe kitarenze amasaha 24, kandi guhagarika ingirabuzimafatizo zabitswe kuri 20 ℃.
Ububiko hamwe nubuzima bwa Shelf
Bibitswe kuri -20 ℃ ± 5 ℃ mu mwijima, byemewe amezi 12.Ubwikorezi buri munsi ya 8 ℃.
