RARA Amabara abiri Kumeneka Uretse Probe
Ibisobanuro:
Dukurikije ihame ryo guhuza ibice fatizo bya ADN, iperereza rya RARA orange na RARA icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru y’imiterere ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.
Ibisobanuro
Dukurikije ihame ryo guhuza ibice fatizo bya ADN, iperereza rya RARA orange na RARA icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru y’imiterere ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.
Ibigize ibikoresho
| Ibigize | Ibisobanuro | ||
| Ibizamini 5 | Ibizamini 10 | Ibizamini 20 | |
| RARA Amabara abiri Kumeneka Uretse Probe | 50μl | 100μl | 200μl |
Porogaramu
Iki gikoresho gikoreshwa kugirango hamenyekane neza imiterere idasanzwe ya gene ya RARA yabantu mu cyitegererezo cya cytologiya cyangwa ingero za paraffine zashyizwe muri vitro.
Kugaragaza ibisabwa
-Icyitegererezo
Ingero za paraffin zashyizwemo ingero zashyizwe muri 10% itagira aho ibogamiye, kandi uburebure bwibice bwari hagati ya 3 na 5 µm.
-Ingero z'akagari
Kubijyanye n'amagufwa cyangwa amaraso ya peripheri, icyitegererezo gishya kidakwiye kubikwa kuri 4 ° C mugihe kitarenze amasaha 24, kandi guhagarika ingirabuzimafatizo bigomba kubikwa kuri -20 ° C.
Ububiko hamwe nubuzima bwa Shelf
Bibitswe kuri -20 ℃ ± 5 ℃ mu mwijima, byemewe amezi 12.Ubwikorezi buri munsi ya 8 ℃.
