Ubushinwa Ibibabi byibihingwa Direct PCR wongeyeho kit-UNG (udafite ibikoresho byo gutoranya) Porotokole Direct PCR ituruka kubakora ibikoresho byinganda nuwabitanze |Foregene

Ibiti byibiti Direct PCR wongeyeho kit-UNG (udafite ibikoresho byo gutoranya) Porotokole Direct PCR ivuye mubikoresho byibimera

Ibibabi byibiti Direct PCR wongeyeho kit-UNG (udafite ibikoresho byo gutoranya) Porotokole Direct PCR ivuye mubikoresho byibimera Byerekanwe

Ibisobanuro:

Koresha mu buryo butaziguye polysaccharide na polifenol amababi ya lysate nk'icyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

◮Nta gutwara igihe kinini kandi gihenze cyo kweza ADN (Igihe, umurimo nigiciro cyazigamye!)

◮Ibikoresho bike

◮Byoroshye - icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya

◮2 X Kuvanga - kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura

◮Byihuse - gutegura inyandikorugero birashobora kurangira muminota 10, PCR reaction irashobora kurangira muminota 50

◮Ibicuruzwa byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Ukurikije ibibabi byibimera Direct PCR Plus, dUTP ikoreshwa mu mwanya wa dTTP, kandi enzyme ya UNG ishobora gutesha agaciro inyandikorugero irimo dUTP yongeyeho.Muri ubu buryo, umwihariko nukuri kwokwongerwaho imbaraga birashimangirwa, kandi ikibazo cyo kwanduza ibicuruzwa bya PCR gishobora kubaho mugihe kinini cyo gupima genetike kirakumirwa.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Buffer P1
	Buffer P2
	6×Ububiko bwa ADN
Igice cya II	2× Ibibabi PCR Biroroshye^TMKuvanga (UNG)

Ibiranga ibyiza

■ Ntabwo bitwara igihe kandi bihenze bya ADN
Material Ibikoresho bike
■ Byoroheje-icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
■ 2 × Kuvanga, kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura
Preparation Imyiteguro yihuse-yuzuye irashobora kurangira muminota 10, PCR reaction irashobora kurangira byibuze muminota 50
■ Ibisubizo byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR

Ibipimo by'ibikoresho

Gusaba: kumenyekanisha genetike, kumenyekanisha genotyping, nibindi.
Icyitegererezo: ikibabi cyibiti
Igipimo: Diameter 2-3mm (uburyo butaziguye), diameter 5-7mm (uburyo bwa lysis)
Urwego rwo gutahura: igice cyintego ≤1kb