Imbuto y'Ibihingwa by'Ubushinwa (Polysaccharide Polyphenol ikungahaye Ntoya) Direct PCR Yongeyeho Kit I (idafite ibikoresho by'icyitegererezo) Ihingura nuwitanga |Foregene

Imbuto y'ibihingwa (Polysaccharide Polyphenol ikungahaye Ntoya) Direct PCR Yongeyeho Kit I (idafite ibikoresho by'icyitegererezo)

Ibisobanuro:

Koresha mu buryo butaziguye imbuto ya polysaccharide polifenol lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

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Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Iki gicuruzwa gikoresha uburyo bwihariye bwa lysis reaction, bushobora kurekura byihuse ADN ya genomique yintangarugero yimbuto yibihingwa hamwe na polysaccharide nyinshi na polifenole (nk'imineke, ipamba, nibindi) kugirango ikoreshwe mubitekerezo bya PCR, bityo rero irakwiriye cyane cyane kwipimisha rinini.Kugirango uhuze ibikenewe mu bizamini bitandukanye byo gutahura, iki gikoresho kirashobora gukoresha ubwoko butandukanye bwintangarugero (nkimbuto zose, gukata uduce duto, cyangwa ingero zubutaka bwimbuto nyinshi) mubushakashatsi.Muri byo, kuburugero rwimbuto rugikeneye kumera, urashobora guhitamo guca uduce duto duto (1-5mg) usibye insoro zimbuto kugirango zigerageze;kubushakashatsi busaba icyitegererezo no kwipimisha mumubare munini wintangarugero, urashobora guhitamo kuvanga ingero nyinshi hanyuma ukazihuza.Nyuma yo gusya mubice bifite diameter ya 0.5mm, ubushakashatsi burashobora gukorwa (byibuze 0.1% byurugero rwicyitegererezo birashobora kuboneka).

Ibisobanuro

50T, 200T, 500T, 2000T

Ibigize ibikoresho

Imbuto y'ibihingwa Direct PCR Yongeyeho Kit I. Polysaccharide Imbuto yibihingwa bya polifenol (Ntoya) Direct PCR Kit
Ibigize ibikoresho		TP-0315T	TP-03151	TP-03152	TP-03153
Ibigize ibikoresho		50 T.	200 T.	500 T.	2000 T.
Igice I.	Buffer SSP1	4.5ml	18ml	45ml	90ml × 2
	Buffer SP2	3ml	10ml	25ml	100ml
	6 × ADN Yipakurura Buffer	1.5ml	1.5ml	1.5ml	1.5ml × 4
Igice cya II	Buffer SSP2	1.5ml	6ml	15ml	60ml
Igice cya II	2 × Imbuto PCR byoroshye^TMKuvanga	500μl	1ml × 2	1.7ml × 3	1.7ml × 12
Igitabo		1	1	1	1