Ubushinwa Ibabi ryibabi rya PCR ibikoresho bitagira ibikoresho (bidafite ibikoresho byo gutoranya) Porotokole Direct PCR ituruka kubakora ibihingwa n’ibicuruzwa |Foregene

Ibibabi byibiti bitaziguye PCR (idafite ibikoresho byo gutoranya) Porotokole Direct PCR ivuye mubikoresho byibimera

Ikibabi cyibiti bitaziguye PCR kitarimo (udafite ibikoresho byo gutoranya) Porotokole Direct PCR ivuye mubikoresho byibimera byerekanwe

Ibisobanuro:

Koresha mu buryo butaziguye amababi cyangwa amababi lysate nk'icyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.

◮Nta gutwara igihe kinini kandi gihenze cyo kweza ADN (Igihe, umurimo nigiciro cyazigamye!)

◮Ibikoresho bike

◮Byoroshye - icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya

◮2 X Kuvanga - kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura

◮Byihuse - gutegura inyandikorugero birashobora kurangira muminota 10, PCR reaction irashobora kurangira muminota 50

◮Ibicuruzwa byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR

Ohereza imeri kuri twe Kuramo nka PDF

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Iki gicuruzwa gikoresha sisitemu idasanzwe ya lysis buffer kugirango amababi yibihingwa.Lysate irashobora gukoreshwa nkicyitegererezo nta kweza.Nyuma yo kongeramo primers, PCR ivanze yiki gikoresho irashobora gukoreshwa muri amplification.

Ibisobanuro

0 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Buffer P1
	Buffer P2
	6×Ububiko bwa ADN
Igice cya II	2× Ibibabi PCR Biroroshye^TMKuvanga

Ibiranga ibyiza

■ Ntabwo bitwara igihe kandi bihenze bya ADN
Material Ibikoresho bike
■ Byoroheje-icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
■ 2 × Kuvanga, kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura
Preparation Imyiteguro yihuse-yuzuye irashobora kurangira muminota 10, PCR reaction irashobora kurangira byibuze muminota 50
■ Ibisubizo byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR

Ibipimo by'ibikoresho

Gusaba: kumenyekanisha genetike, kumenyekanisha genotyping, nibindi.
Icyitegererezo: ikibabi cyibiti
Igipimo: Diameter 2-3mm (uburyo butaziguye), diameter 5-7mm (uburyo bwa lysis)
Urwego rwo gutahura: igice cyintego ≤1kb