Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent (zebrafish)
Ibisobanuro:
Koresha mu buryo butaziguye zebrafish lysate nk'icyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.
◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.
◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.
◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
Ibisobanuro
Iki gicuruzwa gikoresha sisitemu yihariye ya lysis kugirango irekure vuba ADN genomic muri zebrafish nizindi nyama zamafi yamazi meza, amababa yumurizo cyangwa amagi y amafi byerekana PCR, bityo rero birakwiriye cyane cyane kwipimisha geneti nini.Igikorwa cyo kurekura ADN genomic muri buffer ya lysis kirangiye muminota 10-30 kuri 65 ° C.Ntayindi nzira nka protein na RNA ikuraho, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.
2 × PCR BiroroshyeTMKuvanga bifite kwihanganira bikomeye PCR yerekana inhibitor, kandi irashobora gukoresha lysate yicyitegererezo kugirango igeragezwe nkicyitegererezo cyo gukora neza kandi cyihariye.Iyi reagent irimo ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction ya reaction, optimizer ya PCR na stabilisateur.Ikoreshwa ifatanije na lysis buffer irashobora kubona byihuse kandi byoroshye ingero, kandi ifite ibiranga sensibilité yo hejuru, umwihariko ukomeye, hamwe no guhagarara neza.
D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.
Ibisobanuro
50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns
Ibigize ibikoresho
| Igice cya I. | Buffer FP |
| Foregene Protease | |
| 6 × ADN Yipakurura Buffer | |
| Igice cya II | 2 × PCR BiroroshyeTMKuvanga |
| Amabwiriza | |
Ibiranga ibyiza
■Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.
Require Icyitegererezo gikenewe ni gito, nkibishobora 1mg amafi ya caudal fin cyangwa amagi 10 y amafi arashobora gupimwa.
■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
Ibipimo by'ibikoresho
Gushyira mu bikorwa: Amafi ya Zebra nandi mafi yamazi meza.
ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.
Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.
Urujya n'uruza rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.
Buffer FP reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.
v Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.
vReagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.
Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.
vReagent 2 × PCR EasyTM ivanze, niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ kubikwa igihe gito (koresha muminsi 10).
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