ForeSNP Genotyping Kit
Ibisobanuro:
Ukuri kwinshi: kwandika igenzura ryiza, igipimo cyukuri kiri hejuru ya 98%.
Igiciro gito: Ntibikenewe guhuza umubare munini wibiciro bibiri byanditseho probe.
Sisitemu nziza ya PCR: sisitemu nziza itajegajega hamwe na amplification yihariye.
Sisitemu yo kurwanya umwanda PCR: Irashobora gukuraho neza umwanda wa aerosol uterwa n’ibicuruzwa bya PCR, utitaye ku kwivanga kw’ibidukikije ku kimenyetso cya fluorescent cyo kugenzura nabi, kandi bikareba umwihariko wo kongera imbaraga no kwandika neza.
Ibisobanuro
Kurushanwa Allele yihariye PCR (Irushanwa rya Allele yihariye PCR) ni ubwoko bushya bwubwoko bwa allele.Ubu buryo ntibukeneye guhuza ubushakashatsi bwihariye kuri buri SNP na inDel, ariko bukenera gusa ibice bibiri byubushakashatsi bwihariye kugirango bigere ku myandikire yukuri ya ADN ya ADN.Mugusesengura ubukana nigipimo cyikimenyetso cya nyuma cya fluorescence, genotype ihita igenwa, kandi ingaruka zo guhuriza hamwe zigaragara.Ubu buryo bufite igihe gito cyo gutahura, igiciro gito cya reagent, kumenya neza neza, kandi birashobora gukoreshwa mubwoko bwa molekile bwifashishwa mu bworozi, QTL ihagaze, kumenyekanisha ibimenyetso bya genetike hamwe nubundi bushakashatsi bwibinyabuzima bwa molekuline hamwe nubunini bw'icyitegererezo.
Ibisobanuro
5ml, 50ml, 50ml × 10, 500ml × 20
Ibigize ibikoresho
| 2 × GT BiroroshyeTMKuvanga |
| DNase Yubusa ddH2O |
| Amabwiriza |
Ibiranga ibyiza
■Ukuri kwinshi: kwandika igenzura ryiza, igipimo cyukuri kiri hejuru ya 98%.
Cost Igiciro gito: Ntibikenewe guhuza umubare munini wibiciro bibiri-byanditseho probe.
■ Optimized PCR sisitemu: sisitemu nziza itajegajega hamwe na amplification yihariye.
System Sisitemu yo kurwanya umwanda PCR: Irashobora gukuraho neza umwanda wa aerosol uterwa n’ibicuruzwa bya PCR, utitaye ku kwivanga kw’ibidukikije ku kimenyetso cya fluorescent cyo kugenzura nabi, kandi bikareba umwihariko wo kongera imbaraga no kwandika neza.
Gukoresha ibikoresho
Yagenewe gukoreshwa mugupima genotyping ya ADN isukuye.
Urugero
Muri ubu bushakashatsi, ADN ya 200ng ingano yakoreshejwe nk'icyitegererezo kugirango hamenyekane ubwoko bwa TaGS-D1 bujyanye n'uburemere bw'ingano bw'ingano munsi ya Foresnp Genotyping Kit.Icyitegererezo cyibikoresho ni BIO-RAD CFX ihuza;umubare w'icyitegererezo ni 21, umubare wa NTC ni 8, kandi buri bwoko bwo kugenzura neza ni 1.
ForeSNP Genotyping Kit
