Imbuto y'Ibihingwa by'Ubushinwa (Polysaccharide Polyphenol ikungahaye, ntoya) Direct PCR Plus Kit I-UNG (idafite ibikoresho by'icyitegererezo) Ihingura n'utanga isoko |Foregene

Imbuto y'ibihingwa (Polysaccharide Polyphenol ikungahaye, ntoya) Direct PCR Yongeyeho Kit I-UNG (idafite ibikoresho by'icyitegererezo)

Imbuto y'Ibimera (Polysaccharide Polyphenol ikungahaye, ntoya) Direct PCR Yongeyeho Kit I-UNG (idafite ibikoresho by'icyitegererezo) Ishusho Yerekanwe

Ibisobanuro:

Koresha mu buryo butaziguye imbuto ya polysaccharide polifenol lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

- Nta gutwara igihe kandi bihenze byo kweza ADN.

- Icyitegererezo gisabwa ni gito, fata imbuto imwe.

- Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

- Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

- Sisitemu yo kurwanya umwanda PCR 2 × Imbuto PCR EasyTM ivanze (UNG), ishobora gukuraho neza umwanda uterwa n’ibicuruzwa bya PCR kandi ikemeza umwihariko n’ukuri kwa amplification.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Iki gicuruzwa gikoresha uburyo bwihariye bwa lysis reaction, bushobora kurekura byihuse ADN ya genomique yintangarugero yimbuto yibihingwa hamwe na polysaccharide nyinshi na polifenole (nk'imineke, ipamba, nibindi) kugirango ikoreshwe mubitekerezo bya PCR, bityo rero irakwiriye cyane cyane kwipimisha rinini.Kugirango uhuze ibikenewe mu bizamini bitandukanye byo gutahura, iki gikoresho kirashobora gukoresha ubwoko butandukanye bwintangarugero (nkimbuto zose, gukata uduce duto, cyangwa ingero zubutaka bwimbuto nyinshi) mubushakashatsi.Muri byo, kuburugero rwimbuto rugikeneye kumera, urashobora guhitamo guca uduce duto duto (1-5mg) usibye insoro zimbuto kugirango zigerageze;kubushakashatsi busaba icyitegererezo no kwipimisha mumubare munini wintangarugero, urashobora guhitamo kuvanga ingero nyinshi hanyuma ukazihuza.Nyuma yo gusya mubice bifite diameter ya 0.5mm, ubushakashatsi burashobora gukorwa (byibuze 0.1% byurugero rwicyitegererezo birashobora kuboneka).

Ibisobanuro

50T, 200T, 500T, 2000T

Ibigize ibikoresho

Imbuto y'ibihingwa Direct PCR Yongeyeho Kit I-UNG Polysaccharide Imbuto y'Ibimera bya Polifenol (Ntoya) PCR Kit-UNG
Ibigize ibikoresho		TP-0316T	TP-03161	TP-03162	TP-03163
Ibigize ibikoresho		50 T.	200 T.	500 T.	2000 T.
Igice I.	Buffer SSP1	4.5ml	18ml	45ml	90ml × 2
	Buffer SP2	3ml	10ml	25ml	100ml
	6 × ADN Yipakurura Buffer	1.5ml	1.5ml	1.5ml	1.5ml × 4
Igice cya II	Buffer SSP2	1.5ml	6ml	15ml	60ml
Igice cya II	2 × Imbuto PCR EasyTM ivanze	500μl	1ml × 2	1.7ml × 3	1.7ml × 12
Igitabo		1	1	1	1

Ibiranga ibyiza

- Nta gutwara igihe kandi bihenze byo kweza ADN.

- Icyitegererezo gisabwa ni gito, fata imbuto imwe.

- Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

- Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

- Kurwanya umwanda PCR sisitemu 2 × Imbuto PCR Byoroshye^TM Kuvanga (UNG), bishobora gukuraho neza umwanda uterwa nibicuruzwa bya PCR no kwemeza umwihariko nukuri kwa amplification.