Inyamaswa miRNA Kwigunga Kit MicroRNA Gukuramo no Kweza Ibikoresho
Ibisobanuro:
Byihuse kandi e ffi byoroshye gukuramo uduce duto twa RNA ya 20-200nt miRNA, siRNA, snRNA mubice bitandukanye byinyamanswa na selile.
◆Inzira yose ikorerwa mubushyuhe bwicyumba (15-25 ° C.), udafite ubwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
◆Ibikoresho byose byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
◆Inkingi ya ADN-Isukura ihuza ADN byumwihariko, kugirango ibikoresho bishobore gukuraho ADN yanduye utiriwe wongeraho DNase.
◆Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
◆Umuvuduko wihuse: Igikorwa kiroroshye kandi kirashobora kurangira muminota 30.
◆Umutekano: nta gukuramo organic reagent bisabwa.
◆Ubwiza buhanitse: Ibice bya RNA byakuweho bifite isuku nyinshi, bitarimo poroteyine n’indi myanda ihumanya, kandi birashobora kuzuza ibisabwa byamanuka.
◆Porogaramu zitandukanye zigerageza
Ibisobanuro
Iki gikoresho gikoresha spin inkingi hamwe na formula yakozwe na societe yacu, ishobora gukuramo neza uduce duto twa RNA nka 20-200nt miRNA, siRNA, snRNA mubice bitandukanye byinyamanswa na selile.Igikoresho gitanga ADN-Isukura Inkingi, ishobora gutandukanya byoroshye ndengakamere na lysate ya tissue ikanakuraho ADN na RNA ndende kurugero, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA hamwe na formula idasanzwe, Irashobora gutunganya umubare munini wintangarugero icyarimwe.Sisitemu yose ni RNase-Yubusa, kugirango miRNA isukuye ntisuzugurwe;Buffer miRW1, Buffer miRW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.
Ibigize ibikoresho
| Inyamaswa miRNA Kwigunga Kit | ||||
| Ibirimo | RE-0101T | RE-01011 | RE-01012 | RE-01014 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miRL * | 3ml | 25ml | 50ml | 100ml |
| Buffer miRW1 | 4ml | 35ml | 70ml | 140ml |
| Buffer miRW2 | 2.4ml | 24ml | 48ml | 96ml |
| Ubusa ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| Inkingi ya RNA gusa | 5 Gushiraho | 50Gushiraho | 100Gushiraho | 200Gushiraho |
| Inkingi ya ADN | 5 Gushiraho | 50Gushiraho | 100Gushiraho | 200Gushiraho |
| IFU | 1 igice | 1 igice | 1 igice | 1 igice |
Ibiranga ibyiza
◆Inzira yose ikorerwa mubushyuhe bwicyumba (15-25 ° C.), udafite ubwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
◆Ibikoresho byose byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
◆Inkingi ya ADN-Isukura ihuza ADN byumwihariko, kugirango ibikoresho bishobore gukuraho ADN yanduye utiriwe wongeraho DNase.
◆Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
◆Umuvuduko wihuse: Igikorwa kiroroshye kandi kirashobora kurangira muminota 30.
◆Umutekano: nta gukuramo organic reagent bisabwa.
◆Ubwiza buhanitse: Ibice bya RNA byakuweho bifite isuku nyinshi, bitarimo poroteyine n’indi myanda ihumanya, kandi birashobora kuzuza ibisabwa byamanuka.
◆Porogaramu zitandukanye zigerageza
Porogaramu
Birakwiriye gukuramo no kweza miRNA mubice bitandukanye byinyamanswa cyangwa bikonje bikonje.
Ububiko hamwe nubuzima bwa Shelf
Iki gikoresho gishobora kubikwa amezi 24 mugihe cyumutse mubushyuhe bwicyumba (15-25 ° C.), niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri2-8 ° C..Buffer miRL irashobora gushirwa kuri4 ° C.ukwezi 1 nyuma yo kongerahomercaptoethanol(birasabwa kongeramo nonaha).
