RT Byoroshye II (hamwe na gDNase) Master Premix kumurongo wa mbere wa cDNA synthesis ya Real Time PCR hamwe na gDNase
Ibisobanuro:
-Ubushobozi buhagije bwo gukuraho gDNA, ishobora gukuraho gDNA mubishusho muminota 2.
-Uburyo bwiza bwo kwandukura sisitemu, bifata iminota 15 gusa kugirango urangize synthesis yumurongo wambere cDNA.
-Icyitegererezo cyoroshye: inyandikorugero zirimo ibintu byinshi bya GC hamwe nuburyo bugoye bwa kabiri birashobora kandi guhindurwa hamwe nubushobozi buhanitse.
-Ibihe byinshi-byunvikana sisitemu yo kwandukura, pg-urwego rwicyitegererezo nacyo gishobora kubona cDNA nziza.
-Uburyo bwo kwandukura sisitemu ifite ubushyuhe bwinshi, ubushyuhe bwiza ni 42℃, kandi iracyafite imikorere myiza yo kwandukura kuri 50℃.
Ibisobanuro
25 × 20µl rxns, 100 × 20µl rxns
RT BiroroshyeTMII (Hamwe na gDNase) ni sisitemu yo guhinduranya inyandiko yakozwe muburyo bwihariye kugirango PCR ikureho vuba ADN yanduye.5 × gDNase Ivanga irashobora gukuraho vuba genome isigaye muri RNA kuri 42 ° C muminota 2, birinda neza kwivanga kwa genome kubisubizo bya qPCR.
5 × RO-ByoroshyeTMIvanga ririmo Foregene Reverse Transcriptase yakozwe na Foregene, ni ubwoko bushya bwa reverse transcriptase hamwe na RNA ikomeye.Sisitemu yatunganijwe ituma igipimo cyo kwandukura cyihuta, kandi synthesis ya mbere ya cDNA irashobora kuzuzwa kuri 42 ° C muminota 15.
Sisitemu yo kwandukura sisitemu yiki gikoresho irashobora kandi gukomeza ibikorwa byiza kuri 50 ° C.Kubwibyo, nibyiza cyane kubishusho bya RNA bigoye kandi birashobora kwandukura byoroshye inyandikorugero ya RNA hamwe nibintu byinshi bya GC hamwe nuburyo bwisumbuyeho.
Ibigize ibikoresho
| 5 × gDNase ivanze |
| 5 × RO-ByoroshyeTMKuvanga |
| Ubusa ddH2O |
| Amabwiriza |
Ibiranga ibyiza
-Ubushobozi buhagije bwo gukuraho gDNA, ishobora gukuraho gDNA mubishusho muminota 2.
-Uburyo bwiza bwo kwandukura sisitemu, bifata iminota 15 gusa kugirango urangize synthesis yumurongo wambere cDNA.
-Icyitegererezo cyoroshye: inyandikorugero zirimo ibintu byinshi bya GC hamwe nuburyo bugoye bwa kabiri birashobora kandi guhindurwa hamwe nubushobozi buhanitse.
-Ibihe byinshi-byunvikana sisitemu yo kwandukura, pg-urwego rwicyitegererezo nacyo gishobora kubona cDNA nziza.
-Uburyo bwo kwandukura sisitemu ifite ubushyuhe bwinshi, ubushyuhe bwiza ni 42℃, kandi iracyafite imikorere myiza yo kwandukura kuri 50℃.
Porogaramu
Byakoreshejwe muburyo nyabwo PCR kugirango isesengure mu buryo bwuzuye imvugo ya gene.
Irashobora gusesengura byihuse kandi neza inzira ya RNA nka virusi ya RNA.
Guhindura inyandiko mvugo ya RNA ifite ibintu byinshi bya GC cyangwa urwego rwisumbuyeho.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Igikoresho kigomba kubikwa kuri -20° C..Bika ibicuruzwa muri firigo ihoraho yubushyuhe kuri -20 ° C ako kanya ukimara kuyakira.Niba uburyo bwo kubika bukwiye, ibicuruzwa ntibishobora gutesha agaciro imikorere iyo ari yo yose mugihe cyumwaka 1.
