Igikoresho cyo gukuramo Gel ADN Ikuramo ibikoresho
Ibisobanuro:
Byihuse kandi e ffi byoroshye kugarura ibice 20bp-10kb bya ADN biva muri gel ya agarose.
◮Umubare munini wo gukira ADN:Ibice bya ADN bigufi nka 30bp kandi binini nka 10kb birashobora kugarurwa.
◮Uburyo bwiza bwo gukira:Uburyo bwiza bwo gukira bushobora kugera hejuru ya 80%.
◮Sisitemu ntoya:byibuze igisubizo cya 30μl gishobora gukoreshwa mugukuraho, gishobora kongera neza kwibumbira hamwe kwa ADN yagaruwe.
◮Umuvuduko wihuse:Byoroshye gukora, kugarura ibice bya ADN birashobora kurangira muminota 15.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:Ibice bya ADN byagaruwe bifite isuku nyinshi, bishobora guhura nubushakashatsi butandukanye bwakurikiyeho.
Ibisobanuro
Iki gikoresho gikoresha spin na formula yakozwe na societe yacu, ishobora kugarura neza ibice bya ADN bifite isuku nyinshi muri gel ya agarose.Igikoresho kirashobora kugarura ibice bya ADN mubice byinshi.Mubihe bisanzwe, ibice bya ADN ya 30bp-10kb birashobora kugarurwa.Nibura 30μl ya eluent irashobora gukoreshwa kugirango wongere ubunini bwa ADN yagaruwe.
Igikoresho kiroroshye gukora kandi gifite intambwe nke.Irakeneye gusa centrifugation kugirango itunganyirize icyarimwe icyarimwe, kandi ibice byinshi bya ADN byagaruwe neza birashobora kuboneka muminota 15.
Ibisobanuro
50 Imyiteguro
Buffer GE: Kuraho geli kuri 60 ° C kugirango urekure ibice bya ADN muri gel.
Buffer WB1: Kuraho ion zisigaye muri ADN.
Buffer EB: kura ADN kumurongo wo kweza.
ADN-Inkingi Yonyine: Adorption yihariye yibice bya ADN muri gel.
Ibigize ibikoresho
| Buffer GE * |
| Buffer WB1 |
| Buffer EB |
| ADN-Inkingi gusa |
| Amabwiriza |
Ibiranga ibyiza
-Ibice byinshi byo kugarura ADN: ibice bya ADN bigufi nka 30bp kandi binini nka 10kb birashobora kugarurwa.
-Uburyo bwiza bwo gukira: Uburyo bwiza bwo gukira bushobora kugera hejuru ya 80%.
-Gukuraho sisitemu ntoya: byibuze 30μl igisubizo cyo gukuraho gishobora gukoreshwa mugukuraho, gishobora kongera neza kwibumbira mubice bya ADN byagaruwe.
-Umuvuduko wihuse: Byoroshye gukora, kugarura ibice bya ADN birashobora kurangira muminota 15.
-Umutekano: Nta gukuramo reagent ikenewe.
-Ubuziranenge: Ibice bya ADN byagaruwe bifite isuku nyinshi, bishobora guhura nubushakashatsi butandukanye bwakurikiyeho.
Porogaramu
Iki gikoresho kibereye kugarura ibice bya ADN (30bp-10kb) muri geles ya agarose.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C), niba gikeneye kubikwa mugihe kirekire, gishobora kubikwa kuri 2-8 ° C.
Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.
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