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Imbeba Umurizo SuperDirect PCR Kit Direct PCR Lysis Reagent (Umurizo wimbeba) (kuri Genotyping)
Koresha mu buryo butaziguye imbeba umurizo lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.
◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.
◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.
◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
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Inyama zinyamanswa Direct PCR kit-UNG (idafite ibikoresho byo gutoranya) Porotokole
Koresha mu buryo butaziguye inyamanswa yinyamanswa nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.Wongeyeho UNG yo kurwanya umwanda kugirango ikureho ibyiza.
◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.
◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.
◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
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Umurizo wimbeba PCR Kit Direct PCR Lysis Reagent (Umurizo wimbeba) (kuri Genotyping)
Koresha mu buryo butaziguye imbeba umurizo lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.
◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.
◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.
◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
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Umurizo wimbeba PCR Kit-UNG Direct PCR Lysis Reagent (Umurizo wimbeba) (kuri Genotyping)
Imbeba umurizo lysate ikoreshwa muburyo butaziguye nk'icyitegererezo cyo kongera PCR nta gukuramo ADN itandukanye, yihuta kandi yunvikana cyane.Wongeyeho UNG yo kurwanya umwanda kugirango ikureho ibyiza.
◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.
◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.
◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
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Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent (zebrafish)
Koresha mu buryo butaziguye zebrafish lysate nk'icyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.
◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.
◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.
◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
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Ikibabi cyibiti Direct PCR Plus kit (idafite ibikoresho byo gutoranya) Porotokole Direct PCR ivuye mubikoresho byibimera
Koresha mu buryo butaziguye polysaccharide na polifenol amababi ya lysate nk'icyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.
◮Nta gutwara igihe kinini kandi gihenze cyo kweza ADN (Igihe, umurimo nigiciro cyazigamye!)
◮Ibikoresho bike
◮Byoroshye - icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
◮2 X Kuvanga - kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura
◮Byihuse - gutegura inyandikorugero birashobora kurangira muminota 10, PCR reaction irashobora kurangira muminota 50
◮Ibicuruzwa byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR
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Imbuto y'ibihingwa Direct PCR Kit I / II (idafite ibikoresho byo gutoranya) Porotokole Igikoresho cya PCR Ibikoresho
◮Nta gutwara igihe kinini kandi gihenze cyo kweza ADN (Igihe, umurimo nigiciro cyazigamye!)
◮Ibikoresho bike
◮Byoroshye - icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
◮2 X Kuvanga - kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura
◮Byihuse - gutegura inyandikorugero birashobora kurangira muminota 10, PCR reaction irashobora kurangira muminota 50
◮Ibicuruzwa byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR
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Ibiti byibiti Direct PCR wongeyeho kit-UNG (udafite ibikoresho byo gutoranya) Porotokole Direct PCR ivuye mubikoresho byibimera
Koresha mu buryo butaziguye polysaccharide na polifenol amababi ya lysate nk'icyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.
◮Nta gutwara igihe kinini kandi gihenze cyo kweza ADN (Igihe, umurimo nigiciro cyazigamye!)
◮Ibikoresho bike
◮Byoroshye - icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
◮2 X Kuvanga - kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura
◮Byihuse - gutegura inyandikorugero birashobora kurangira muminota 10, PCR reaction irashobora kurangira muminota 50
◮Ibicuruzwa byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR
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RT-qPCR Byoroshyeᵀᴹ (Intambwe imwe) -Taqman
- Umwe-intambwe yintambwe ituma transcript ihinduka na PCR ikorerwa mumiyoboro imwe, gusa ukeneye kongeramo inyandikorugero RNA, primers yihariye ya PCR na ddH yubusa ya RNase.2O.
- Isesengura nyaryo ryuzuye rya virusi ya RNA cyangwa RNA irashobora gukorwa vuba kandi neza.
- Igikoresho gikoresha uburyo bwihariye bwa Foregene revers transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yo gukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
- Sisitemu yogukora neza ituma reaction igira ibyiyumvo byo hejuru byo gutahura, gukomera kwumuriro, no kwihanganira neza.
- RT-qPCR BiroroshyeTM.
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RNAlater (Kubijyanye na RNA) Igisubizo cya RNAlater
Gukosora byihuse inyama zintangarugero zafashwe vuba, kubuza ibikorwa bya RNase, kurinda RNA kwangirika.
-Reagent ikoreshwa muburyo butaziguye, intambwe imwe irahari, kandi RNA ihita itekana kandi ikarindwa.
-Gukorera mubushyuhe bwicyumba, byoroshye, umutekano kandi ntabwo ari uburozi.
-Kugira ngo ukureho ibibazo bya barafu yumye cyangwa firigo yo hasi yubushyuhe bwo kubika tissue.
-Tissue irashobora kubikwa igihe kirekire nta ngaruka zo kwangirika kwa RNA, ikemeza amakuru yizewe yerekana imiterere yamakuru.
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PCR Intwari
◮Ubudahemuka bwo hejuru: 6ibihe bya enzyme isanzwe ya Taq;
◮Byihuse aumuvuduko wa mplification:umuvuduko wa amplification ni 5-10sec / kb, bikubye inshuro 6-12 iy'umusemburo wa Taq usanzwe;
◮Inyandikorugero nyinshi: irashobora kwagura neza inyandikorugero zitandukanye za ADN zifite ibintu byinshi bya GC kandi bigoye kubyongera;
◮HejuruGukora neza: umubare wikizunguruka cyikigereranyo kiri munsi yumusemburo wa Taq usanzwe;
◮Stronger Kwihanganira ibidukikije: yashyizwe kuri 37 ° C icyumweru, ikomeza ibirenze90%ibikorwa;
◮Ifite ibikorwa bya 5 '→ 3' ADN polymerase na 5 '→ 3' ibikorwa bya exonuclease, nta gikorwa cya 3 '→ 5'.
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PCR Byoroshyeᵀᴹ
Sisitemu idasanzwe ya reaction hamwe nubushobozi buhanitse Taq ADN Polymerase ituma PCR reaction ikora neza cyane, amplificateur na sensitivite.
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Terefone
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