Ibinyabuzima bya molekuline reagent Abatanga ninganda |Ubushinwa Molecular Biologiya reagent Ababikora

Ibinyabuzima bya molekuline Reagent

Urukurikirane rwa RNA

Urukurikirane rwa ADN

Urukurikirane rwa RT-qPCR

Amaraso atondekanya PCR

Tera urukurikirane rwa PCR

Amatungo ataziguye ya PCR

Urukurikirane rwa PCR / RT-PCR

IVD ibikoresho fatizo

RT-PCR Byoroshyeᵀᴹ I (Intambwe imwe)

◮Igikoresho kimwe cyintambwe ituma transcription transcription na PCR ikorerwa mumiyoboro imwe.Irakeneye gusa kongeramo inyandikorugero RNA, progaramu yihariye ya PCR na ddH yubusa ya RNase₂O.

◮Isesengura-nyaryo ryuzuye rya RNA rirashobora gukorwa vuba kandi neza.

◮Igikoresho gikoresha uburyo bwihariye bwa Foregene reverse transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yogukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.

◮Sisitemu yogukora neza ituma reaction igira sensibilité yo hejuru, gukomera kwumuriro, no kwihanganira neza.

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Imbeba Umurizo ADN Mini Kit Genomic ADN Ikuramo Ibikoresho biva murizo

Igikoresho cyihuta cyane kwisi gukuramo ADN ya genomic ADN, ishobora gukuramo ADN nziza cyane mumirizo yimbeba mugihe cyisaha 1.

◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.

◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.

◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.

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Bagiteri ya ADN Yigunze Kit Bakteri ya Genomic Gukuramo ibikoresho byo kweza

Byihuse usukure ADN ya genomic yo mu rwego rwo hejuru muri bagiteri mugice cyo gukura kwa logarithmic.

◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.

◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.

◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.

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ATM / CSP11 Amabara abiri

Dukurikije ihame ryo guhuza ibice fatizo bya ADN, iperereza rya ATM orange na CSP11 icyatsi kibisi ryakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru y’imiterere ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.

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ERG1 / TERT Amabara abiri

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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Ibikoresho byo gukuramo ADN / RNA (Amasaro ya Magnetique)

- Inzira yose yo kwigunga ifite umutekano kandi iroroshye

- ADN yakuwe muri selile yubusa ifite umusaruro mwinshi, ubuziranenge bwinshi nubwiza bwizewe

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20q12 / 20q13.33 Amabara abiri

Dukurikije ihame ryo guhuza ibice bya ADN, 20q12 (D20S108) icyatsi cya orange na 20q33.3 icyatsi kibisi cyakoreshejwe mu guhuza hamwe n’intego za ADN zikurikirana, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.

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p53 / D13S319 Amabara abiri

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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EndoFree Maxi Plasmid Kit (Inkingi izunguruka)

Ibikorwa byose byo gukuramo bifata isaha 1 gusa, byemeza imikorere yoroshye kandi yihuse.

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MALT1 Amabara abiri Kumena Uretse iperereza

Dukurikije ihame rya ADN yuzuzanya, MALT1 orange-umutuku na MALT1 icyatsi kibisi byakoreshejwe kugirango bivangwe hamwe na ADN ikurikiranye muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse arasesengurwa kuri microscope ya fluorescence.

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D7S522 / CSP7 Amabara abiri

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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DAPI Kurwanya

Igice cyingenzi cya DAPI yongeye gusiga igisubizo ni 4-fenylindole, ishobora kwinjira mumyanya ngirabuzimafatizo kandi igahuza cyane na ADN.

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