Ibinyabuzima bya molekuline reagent Abatanga ninganda |Ubushinwa Molecular Biologiya reagent Ababikora

Ibinyabuzima bya molekuline Reagent

Urukurikirane rwa RNA

Urukurikirane rwa ADN

Urukurikirane rwa RT-qPCR

Amaraso atondekanya PCR

Tera urukurikirane rwa PCR

Amatungo ataziguye ya PCR

Urukurikirane rwa PCR / RT-PCR

IVD ibikoresho fatizo

Inhibitor ya RNase

◮Imbeba nshya ikomoka kuri RNase inhibitor yerekanwe muri E. coli yakozwe na bagiteri ikoresheje tekinoroji ya gene.Inhibitor ibuza ibikorwa bya RNase mukudahiganwa guhuza na RNase 1: 1, bityo bikarindaRNAubunyangamugayo, kandi burashobora kubuza nezaiRNase A, B, C. ibikorwa, ariko ntabwo RNase T1, T2, H, nibindi.; Ihindurwa ryo guhuza kwa Inhibitori ya RNase na RNase, hamwe nurusobekerane rushobora gutandukanywa na urea na sulfhydryl reagents, kugirango RNase ihindurwe hamwe na inhibitor idakora kuburyo budasubirwaho.

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Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent (zebrafish)

Gukoresha mu buryo butaziguye zebrafish lysate nk'icyitegererezo cyo kongera PCR, nta gukuramo ADN itandukanye, byihuse kandi byoroshye.Wongeyeho UNG yo kurwanya umwanda kugirango ikureho ibyiza.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

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Foreasy Taq ADN Polymerase

◮Umwihariko wo hejuru: Enzyme ifite ibikorwa bishyushye-gutangira.

◮Kwiyongera byihuse: amasegonda 10 / kb.

◮Inyandikorugero ihindagurika cyane: irashobora gukoreshwa muguhindura neza GC agaciro gakomeye, ibintu bitandukanye-bigoye-kwagura inyandikorugero ya ADN.

◮Ubudahemuka bukomeye: Enzyme isanzwe ya Taq inshuro 6.

◮Ubushyuhe bukomeye bwumuriro: Irashobora gushyirwa kuri 37 ° C icyumweru kandi igakomeza ibikorwa birenga 90%.

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Foreasy HS Taq ADN Polymerase

◮Umwihariko wo hejuru: Enzyme hamwe nibikorwa bishyushye-byo gutangira.

◮Kwiyongera byihuse: amasegonda 10 / kb.

◮Inyandikorugero yo hejuru ihindagurika: irashobora gukoreshwa mugukwirakwiza neza HejuruGCagacironabitandukanye bigoye-kwagura inyandikorugero ya ADN.

◮Ubudahemuka bukomeye: Ubudahemuka ni inshuro 6of Enzyme isanzwe.

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Tissue yinyamanswa Direct PCR kit (idafite ibikoresho byerekana) Porotokole

Koresha mu buryo butaziguye inyamanswa yinyamanswa nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

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Igihe nyacyo PCR Byoroshyeᵀᴹ-Taqman

◮Byoroshye - 2 × PCR Kuvanga kugirango ugabanye ikosa ryikigereranyo nigihe cyo gukora

◮Byihariye-byateguwe neza na hot-itangira Taq enzyme irashobora gukumira amplification idasanzwe hamwe na primer dimer

◮Ubukangurambaga bukabije - bushobora kumenya kopi ntoya yicyitegererezo

◮Guhindura byinshi-bihujwe nibikoresho nyabyo-byuzuye bya PCR

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RT Easyᵀᴹ II Master Premix kumurongo wambere wa cDNA synthesis kumwanya nyawo PCR

- Sisitemu nziza yo kwandukura sisitemu, bifata iminota 20 gusa kugirango urangize synthesis yumurongo wambere cDNA.

- Sisitemu yo hejuru-yimikorere ya sisitemu yo kwandukura, pg-urwego rwicyitegererezo na rwo rushobora kubona cDNA nziza.

- Sisitemu yo kwandukura sisitemu ifite ubushyuhe buhanitse, ubushyuhe bwa reaction ya sisitemu irashobora kuba hejuru ya 50 and, kandi ifite imikorere myiza yo kwandukura.

- Ndetse na RNA yanduye (inzoga zigera kuri 60%, umunyu wa guanidine kugeza kuri 750mM) urashobora gukorerwa reaction ya reaction.

- 2 × RT CYANGWA-Byoroshye^TM Kuvanga byongeweho uburyo bwiza bwo guhindura inyandiko (Primer Primer, Oligo (dT)₁₈ Primer).

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Amaraso SuperDirectᵀᴹ PCR Kit-EDTA Amaraso Direct PCR Master Mix ya Genotyping yamaraso

Koresha mu buryo butaziguye EDTA irwanya amaraso yose nk'icyitegererezo cyo kongera PCR, hamwe na sensibilité nyinshi kandi ikora neza.

◮Ntibikenewe koza ADN bitwara igihe kandi bihenze, nta kwitegura, no gukoresha amaraso yose nkicyitegererezo cya PCR.

◮Sisitemu ifite ubushobozi bwo kongera imbaraga kandi irashobora kumenya neza ibice bigize ADN bigizwe na genome.

◮2 × Byoroshye^TMKuvanga-EDTA Kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.

◮Icyitegererezo gifunze byuzuye, ntabwo rero bikenewe guhangayikishwa nicyitegererezo cyanduye nibisubizo byiza bya PCR.

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Intwari ya PCR (Hamwe n'irangi)

◮Ubudahemuka buhebuje: inshuro 6 z'umusemburo wa Taq usanzwe;

◮Umuvuduko wihuse

◮Inyandikorugero nyinshi

◮Gukora neza cyane

◮Kwihanganira ibidukikije birakomeye: bishyirwa kuri 37 ° C icyumweru, bikomeza ibikorwa birenga 90%;

◮Ifite ibikorwa bya 5 '→ 3' ADN polymerase na 5 '→ 3' ibikorwa bya exonuclease, nta gikorwa cya 3 '→ 5'.

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Amaraso SuperDirectᵀᴹ PCR Kit-Heparin Amaraso Direct PCR Master mix ya Genotyping yamaraso

Koresha mu buryo butaziguye heparin anticoagulated maraso yose nkicyitegererezo cyo kongera PCR, hamwe na sensibilité nyinshi kandi ikora neza.

◮Ntibikenewe koza ADN bitwara igihe kandi bihenze, nta kwitegura, no gukoresha amaraso yose nkicyitegererezo cya PCR.

◮Sisitemu ifite ubushobozi bwo kongera imbaraga kandi irashobora kumenya neza ibice bigize ADN bigizwe na genome.

◮2 × Byoroshye^TMKuvanga-Heparin Kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.

◮Icyitegererezo gifunze byuzuye, ntabwo rero bikenewe guhangayikishwa nicyitegererezo cyanduye nibisubizo byiza bya PCR.

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Amaraso SuperDirectᵀᴹ PCR Kit (UNG) -Heparin Amaraso Direct PCR Master mix ya Genotyping yamaraso

Koresha mu buryo butaziguye heparin anticoagulated maraso yose nkicyitegererezo cyo kongera PCR, hamwe na sensibilité nyinshi kandi ikora neza.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

◮Sisitemu ifite ubushobozi bwo kongera imbaraga kandi irashobora kumenya neza ibice bigize ADN bigizwe na genome.

◮2 × Byoroshye^TMKuvanga (UNG) -Heparin Kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.

◮Icyitegererezo gifunze byuzuye, ntabwo rero bikenewe guhangayikishwa nicyitegererezo cyanduye nibisubizo byiza bya PCR.Sisitemu yo kurwanya umwanda PCR

◮2 × Byoroshye^TMKuvanga (UNG), kurandura burundu umwanda uterwa nibicuruzwa bya PCR, kandi urebe neza umwihariko nukuri kwa amplification.

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Amaraso SuperDirectᵀᴹ PCR Kit (UNG) -EDTA Amaraso Direct PCR Master mix ya Genotyping yamaraso

Koresha mu buryo butaziguye EDTA irwanya amaraso yose nk'icyitegererezo cyo kongera PCR, hamwe na sensibilité nyinshi kandi ikora neza.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

◮Ntibikenewe koza ADN bitwara igihe kandi bihenze, nta kwitegura, no gukoresha amaraso yose nkicyitegererezo cya PCR.

◮Sisitemu ifite ubushobozi bwo kongera imbaraga kandi irashobora kumenya neza ibice bigize ADN bigizwe na genome.

◮2 × Byoroshye^TMKuvanga (UNG) -EDTA ifite kwihanganira amaraso menshi: kugeza 45% mumaraso yabantu na 20% mumaraso yimbeba.

◮Icyitegererezo gifunze byuzuye, ntabwo rero bikenewe guhangayikishwa nicyitegererezo cyanduye nibisubizo byiza bya PCR.Sisitemu yo kurwanya umwanda PCR

◮2 × Byoroshye^TMKuvanga (UNG) -EDTA, ikuraho neza umwanda uterwa nibicuruzwa bya PCR, kandi ukemeza umwihariko nukuri kwa amplification.

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