Ubushinwa PCR Intwari (Hamwe Irangi) Ihingura nuwitanga |Foregene

Intwari ya PCR (Hamwe n'irangi)

PCR Intwariᵀᴹ (Hamwe Irangi) Ishusho Yerekanwe

Ibisobanuro:

◮Ubudahemuka buhebuje: inshuro 6 z'umusemburo wa Taq usanzwe;

◮Umuvuduko wihuse

◮Inyandikorugero nyinshi

◮Gukora neza cyane

◮Kwihanganira ibidukikije birakomeye: bishyirwa kuri 37 ° C icyumweru, bikomeza ibikorwa birenga 90%;

◮Ifite ibikorwa bya 5 '→ 3' ADN polymerase na 5 '→ 3' ibikorwa bya exonuclease, nta gikorwa cya 3 '→ 5'.

Ohereza imeri kuri twe Kuramo nka PDF

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

5ml, 10ml, 40ml

PCR Intwari (Hamwe Irangi) Ibikoresho byo gukuramo birimo Foregene idasanzwe idasanzwe ya Taq - Foregene Taq Intwari, optime ya DNTPs, MgCl2, buffer reaction, PCR itera imbaraga, optimizer na stabilisateur.Sisitemu ya 2 × PCR HeroTM ivanze (irangi) ifite kwihanganira cyane inhibitori za PCR kuruta sisitemu isanzwe ya PCR ivanze, kandi irashobora guhangana byoroshye na PCR yogukwirakwiza inyandikorugero zitandukanye.Sisitemu idasanzwe ya reaction hamwe nubushobozi buhanitse Taq Intwari ituma reaction ya PCR igira ubushobozi bwo kongera amplification, umwihariko hamwe na sensitivite.

Ibigize ibikoresho

0.1U Intwari ya Taq /μl

400μM DNTP

20mM Tris-HCl (pH8.8)

100mM KCl

5mM MgCl2

Izindi stabilisateur hamwe nabazamura

Ububiko bwa ADN

Ibiranga ibyiza

■Ubudahemuka buhebuje: inshuro 6 z'umusemburo wa Taq usanzwe;

Speed Umuvuduko wihuse wihuta: umuvuduko wa amplification ni 5-10sec / kb, wikubye inshuro 6-12 ugereranije na Taq enzyme isanzwe;

Ibindi byitegererezo byo guhuza n'imiterere: Irashobora kwagura neza inyandikorugero zitandukanye za ADN hamwe na hejuruIbirimo GC;

Efficiency Gukora neza cyane: kugabanuka kwinshi kurenza imisemburo ya Taq isanzwe;

Ance Kwihanganira ibidukikije birakomeye: bishyirwa kuri 37°C icyumweru, gukomeza ibikorwa birenga 90%;

■ Ifite 5 '→3 'Igikorwa cya ADN polymerase na 5 '→3 'ibikorwa bya exonuclease, nta 3 '→5 'ibikorwa.

Kit Ibikoresho byabanjirijwe kuvangwa na Electrophoresis Loading Buffer, PCR imaze kurangira, icyitegererezo gishobora gutwarwa na electrophorei;imikorere iroroshye kandi yoroshye.