Igihe nyacyo PCR Byoroshyeᵀᴹ-SYBR Icyatsi I kit
Ibisobanuro:
Byoroshye-2X PCR Kuvanga kugirango ugabanye ikosa ryikigereranyo nigihe cyo gukora
Byihariye-byateguwe neza na hot-itangira Taq enzyme irashobora gukumira amplification idasanzwe hamwe na primer dimer
Ubukangurambaga bukabije - bushobora kumenya kopi ntoya yicyitegererezo
Guhindura byinshi-bihujwe nibikoresho nyabyo-byuzuye bya PCR
Ibisobanuro
2 × PCR nyayo byoroshyeTMKuvanga-SYBR yatanzwe nigihe nyacyo PCR ByoroshyeTM-SYBR Icyatsi I kit ni sisitemu nshya ya premix ikoresha SYBR Icyatsi I kuri Real Time PCR amplification reaction, ishobora kuzamura cyane umwihariko wibicuruzwa no kubyumva neza.ROX itangwa nk'irangi ryo kugenzura imbere.Ubwinshi bwa fluorescence yiki gikoresho bwikubye inshuro 3-5 ibyo bicuruzwa bisa, birashobora kuba byoroshye kandi bikagaragaza ubushishozi bwibanze bwa ADN yerekana icyitegererezo.
2 × PCR nyayo yoroshyeTMKuvanga-SYBR irimo Foregene idasanzwe ishyushye-itangira Taq ADN Polymerase.Ugereranije na misemburo isanzwe ya Taq, ifite ibyiza byo gukora neza cyane, ubushobozi bwihariye bwo kongera imbaraga hamwe nigipimo gito kidahuye.Irashobora kugabanya amplification idasanzwe no kunoza ukuri kwa PCR.
Ibisobanuro
| Igihe nyacyo PCR Byoroshyeᵀᴹ-SYBR Icyatsi I Kit | ||||
| Ibigize ibikoresho (sisitemu ya 20μl) | QP-01011 | QP-01012 | QP-01013 | QP-01014 |
| 200T | 500T | 1000T | 2000T | |
| 2 × PCR nyayo yoroshyeTMKuvanga-SYBR | 1 ml × 2 | 1,7 ml × 3 | 1,7 ml × 6 | 1,7 ml × 12 |
| 50 × ROX Yerekana irangi | 400 μl | 1 ml | 1 ml × 2 | 1 ml × 4 |
| DNase Yubusa ddH2O | 1,7 ml | 1,7 ml × 2 | 10 ml | 20 ml |
| Amabwiriza | 1 | 1 | 1 | 1 |
Ibiranga ibyiza
Byoroshye - 2X PCR Kuvanga kugirango ugabanye ikosa ryikigereranyo nigihe cyo gukora
■ Byihariye - byateguwe neza na hot-itangira Taq enzyme irashobora gukumira amplification idasanzwe hamwe na primer dimer
■ Ibyiyumvo bihanitse - birashobora kumenya kopi nkeya yicyitegererezo
Ver Guhindura byinshi-bihujwe nibikoresho nyabyo-byuzuye bya PCR
Porogaramu
Isesengura rya QPCR
Urujya n'uruza rw'akazi
Igishushanyo
Ububiko nubuzima bwiza
Iki gikoresho kigomba kubikwa kure yumucyo kandi kigomba kubikwa kuri -20 ℃.Niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ mugihe gito (iminsi 10).
