Ibinyabuzima bya molekuline reagent Abatanga ninganda |Ubushinwa Molecular Biologiya reagent Ababikora

Ibinyabuzima bya molekuline Reagent

Urukurikirane rwa RNA

Urukurikirane rwa ADN

Urukurikirane rwa RT-qPCR

Amaraso atondekanya PCR

Tera urukurikirane rwa PCR

Amatungo ataziguye ya PCR

Urukurikirane rwa PCR / RT-PCR

IVD ibikoresho fatizo

MYC / IGH Amabara abiri

Ukurikije ihame rya ADN yuzuzanya, iperereza rya MYC orange-umutuku na IGH icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.

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Igikoresho cyo gukuramo Gel ADN Ikuramo ibikoresho

Byihuse kandi e ﬃ byoroshye kugarura ibice 20bp-10kb bya ADN biva muri gel ya agarose.

◮Umubare munini wo gukira ADN:Ibice bya ADN bigufi nka 30bp kandi binini nka 10kb birashobora kugarurwa.

◮Uburyo bwiza bwo gukira:Uburyo bwiza bwo gukira bushobora kugera hejuru ya 80%.

◮Sisitemu ntoya:byibuze igisubizo cya 30μl gishobora gukoreshwa mugukuraho, gishobora kongera neza kwibumbira hamwe kwa ADN yagaruwe.

◮Umuvuduko wihuse:Byoroshye gukora, kugarura ibice bya ADN birashobora kurangira muminota 15.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:Ibice bya ADN byagaruwe bifite isuku nyinshi, bishobora guhura nubushakashatsi butandukanye bwakurikiyeho.

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1q21 Ikirangantego Orange Ikibazo

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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D13S25 Spectrum Orange Ikibazo

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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CCND1 / IGH Amabara abiri Amabara abiri yo guhuza

Ukurikije ihame rya ADN yuzuzanya, ubushakashatsi bwa CCND1 orange-umutuku na IGH icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.

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RB1 / 1q21 Amabara abiri

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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HER2 / CSP17 Amabara abiri

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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ETV6 Ibice bibiri Kumena Ibice Bitandukanye

Dukurikije ihame fatizo ryuzuzanya rya ADN, iperereza rya ETV6 orange-umutuku na ETV6 icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.

iperereza burambuye
D13S319 Spectrum Orange Ikibazo

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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P16 / CSP3 / CSP17 / CSP7 Amabara abiri

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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RARA Amabara abiri Kumeneka Uretse Probe

Dukurikije ihame ryo guhuza ibice fatizo bya ADN, iperereza rya RARA orange na RARA icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru y’imiterere ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.

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IGH Amabara abiri Kumena Uretse Probe

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

iperereza burambuye