Ibinyabuzima bya molekuline reagent Abatanga ninganda |Ubushinwa Molecular Biologiya reagent Ababikora

Ibinyabuzima bya molekuline Reagent

Urukurikirane rwa RNA

Urukurikirane rwa ADN

Urukurikirane rwa RT-qPCR

Amaraso atondekanya PCR

Tera urukurikirane rwa PCR

Amatungo ataziguye ya PCR

Urukurikirane rwa PCR / RT-PCR

IVD ibikoresho fatizo

Imbuto y'ibihingwa (Polysaccharide Polyphenol ikungahaye, ntoya) Direct PCR Yongeyeho Kit I-UNG (idafite ibikoresho by'icyitegererezo)

Koresha mu buryo butaziguye imbuto ya polysaccharide polifenol lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

- Nta gutwara igihe kandi bihenze byo kweza ADN.

- Icyitegererezo gisabwa ni gito, fata imbuto imwe.

- Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

- Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

- Sisitemu yo kurwanya umwanda PCR 2 × Imbuto PCR EasyTM ivanze (UNG), ishobora gukuraho neza umwanda uterwa n’ibicuruzwa bya PCR kandi ikemeza umwihariko n’ukuri kwa amplification.

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Imbuto y'ibihingwa (binini kandi biciriritse) Direct PCR Yongeyeho Kit II-UNG (idafite ibikoresho by'icyitegererezo)

Koresha mu buryo butaziguye imbuto ya polysaccharide polifenol lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

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Imbuto y'ibihingwa (Polysaccharide Polyphenol ikungahaye Ntoya) Direct PCR Yongeyeho Kit I (idafite ibikoresho by'icyitegererezo)

Koresha mu buryo butaziguye imbuto ya polysaccharide polifenol lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

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Imbuto y'ibihingwa (Polysaccharide Polyphenol ikungahaye, nini kandi nini-nini) Direct PCR Plus Kit II-UNG (idafite ibikoresho by'icyitegererezo)

Koresha mu buryo butaziguye imbuto ya polysaccharide polifenol lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

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Imbuto y'ibihingwa (Kinini) Direct PCR Kit II-UNG (idafite ibikoresho by'icyitegererezo)

Gukoresha mu buryo butaziguye imbuto lysate nk'icyitegererezo cyo kongera PCR, nta gukuramo ADN itandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.

◮Icyitegererezo gisabwa ni gito, fata imbuto imwe.

◮Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

◮Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

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Icyerekezo Cyerekezo RT-qPCR Kit

◮Kuri RNA itaziguye-nyayo-nyayo kuva muri swab yakusanyije idafite inzira yo kweza RNA.Iki gikoresho cyuzuza qRT-PCR mugihe cyisaha imwe.Enzyme ivanze nuruvange rwiza rwa Reverse Transcriptase, Hot-Tangira Taq ADN polymerase, RNase inhibitor.Buffer ya Reaction ikubiyemo ibice byose bisabwa, harimo ibice byahinduwe neza, Mg²⁺, DUTP, na DNTP.

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PCR Isukura Kit PCR Isukura Sisitemu ya Ultra-yera

Byihuse usukure kandi ubone ibice bya ADN byujuje ubuziranenge muri sisitemu ya PCR;niba ushaka gutandukana no kubona ibice bya ADN bya ADN, nyamuneka hitamo ibikoresho byo kugarura gel.

Range Urwego runini rwo kugarura ADN: Ibice bya ADN bigufi nka 60bp kandi binini nka 10kb birashobora kugarurwa.

Performance Gukora neza: Gukora neza muri rusange birenze 80%, kandi hejuru irashobora kugera hejuru ya 95%.

Speed Umuvuduko wihuse:Biroroshye gukora, kugarura ibice bya ADN birashobora kurangira muminota 15 kandi primer dimer irashobora gukurwaho bitabaye ngombwa gukira gel.

◮ S.umutekano: Nta gukuramo organic reagent bisabwa.

Quality Ubwiza bwo hejuru: Ibice bya ADN byagaruwe bifite isuku nyinshi, bishobora guhura nubushakashatsi butandukanye bwakurikiyeho.

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Intebe ya ADN Yigunze Igikoresho cya ADN Ikuramo Ibikoresho byoza

Byihuse usukure kandi ubone ADN ya genomic yo mu rwego rwo hejuru uhereye ku ntebe zitandukanye.

◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.

◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.

◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.

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Icyitegererezo cyo Kurekura

◮Byoroshye gukora:icyitegererezo lysis mugihe cyo gukusanya icyitegererezo

AstByihuse kandi byoroshye:Ntibikenewe gukuramo aside nucleic, ibereye gutahura nini

ScopeUrwego rwagutse rwo gusaba:PCR mu buryo butaziguye nyuma yo gukusanya icyitegererezo

Umutekano kandi wizewe:Kora vuba vuba virusi, garanti umutekano kurwego runini

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Ubutaka ADN Yigunze Ibikoresho byo gukuramo ibikoresho byo kwisukura & Reagents kubutaka bwa ADN

Byihuse usukure kandi ubone ADN yo mu rwego rwohejuru ya mikorobe itandukanye yubutaka.

◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.

◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.

◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.

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FFPE ADN Yigunze Igikoresho cyo gukuramo ADN yo gukuramo ibikoresho bya FFPE

Byihuse usukure ADN yo mu rwego rwo hejuru ivuye mu bice byashyizwemo ibice nka para ﬃ n ibice na para ﬃ n bice.

◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.

◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.

◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.

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RT-PCR Byoroshyeᵀᴹ II (Intambwe ebyiri)

◮Isesengura-nyaryo ryuzuye rya RNA rirashobora gukorwa vuba kandi neza.

◮Igikoresho gikoresha uburyo bwihariye bwa Foregene reverse transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yogukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.

◮ Sisitemu yogukora neza ituma reaction igira sensibilité yo hejuru, gukomera kwumuriro, no kwihanganira neza.

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