Icyerekezo Cyerekezo RT-qPCR Kit
Ibisobanuro:
◮Kuri RNA itaziguye-nyayo-nyayo kuva muri swab yakusanyije idafite inzira yo kweza RNA.Iki gikoresho cyuzuza qRT-PCR mugihe cyisaha imwe.Enzyme ivanze nuruvange rwiza rwa Reverse Transcriptase, Hot-Tangira Taq ADN polymerase, RNase inhibitor.Buffer ya Reaction ikubiyemo ibice byose bisabwa, harimo ibice byahinduwe neza, Mg2+, DUTP, na DNTP.
Ibisobanuro
ForeDirect RT-qPCR Kit yateguwe kuri RNA itaziguye igihe nyacyo cyo gukusanya ibicuruzwa bivuye muri swab idafite inzira yo kweza RNA.Iki gikoresho cyuzuza qRT-PCR mugihe cyisaha imwe.Enzyme ivanze nuruvange rwiza rwa Reverse Transcriptase, Hot-Tangira Taq ADN polymerase, RNase inhibitor.Buffer ya Reaction ikubiyemo ibice byose bisabwa, harimo ibice byahinduwe neza, Mg2+, DUTP, na DNTP.
Ibisobanuro
100T
Ibigize ibikoresho
| Nucleic aside irekura |
| Kurinda RNA |
| 2 × RT-qPCR Buffer |
| Imvange ya Enzyme (Taq & M-MLV) |
| Amabwiriza |
Porogaramu ya PCR
1. Umuyoboro wa PCR umaze gushyirwaho mugihe gito, ubishyire mubitegererezo by'ibikoresho byongera imbaraga.
2. Porotokole yubushyuhe
| Intambwe | Ubushyuhe | Igihe | Amagare | |
| 1 | Guhindura inyandiko | 50 ℃ | Iminota 15 | 1 |
| 2 | Mbere yo gutandukana | 95 ℃ | Imin | 1 |
| 3 | Gutandukana | 95 ℃ | Amasegonda 10 | 42 |
| 4 | Umugereka / Kwagura | 60 ℃ | Amasegonda 30 | |
Icyitonderwa:Ikimenyetso cya Fluorescent cyagaragaye nyuma yintambwe yo kwaguka ya buri cyiciro.
3. Nyuma yo gushiraho, bika dosiye hanyuma ukore progaramu ya reaction.
Intambwe Zikora
Mbere yo gutegura reagent, reagent zose ziri mubikoresho zigomba gukonjeshwa mubushyuhe bwicyumba hanyuma zikavangwa buhoro.
Witegure neza
1. Tegura Nucleic Acide Isohora
| Ibigize | Ingano kuri Sample cyangwa Igenzura |
| Nucleic aside irekura | 5μL |
| Kurinda RNA | 0.5 μL |
2. Tegura ivangavanga
| Ibigize | Ingano kuri Sample cyangwa Igenzura |
| 2 × RT-qPCR Buffer | 15 μL |
| Imvange ya Enzyme (Taq & M-MLV) | 1.5 μL |
| Ibibazo nibibazo | 1μL |
Ububiko hamwe nubuzima bwa Shelf
-Igikoresho kigomba kubikwa kuri -20± 5℃.Igihe cyemewe ni amezi 12.
-Irinde gusubiramo inshuro nyinshi (<5 cycle).
