RT-qPCR Byoroshyeᵀᴹ (Intambwe imwe) -SYBR Icyatsi I.
Ibisobanuro:
◮Igikoresho kimwe cyintambwe ikora transcription transcription na qPCR reaction ebyiri muburyo bumwe, gusa ukeneye kongeramo inyandikorugero RNA, progaramu ya PCR yihariye na RNase-Free ddH2O.
◮Igikoresho kirashobora gusesengura byihuse kandi neza muburyo bwo gusesengura virusi ya RNA cyangwa gukurikirana RNA.
◮Igikoresho gikoresha uburyo bwihariye bwa Foregene reverse transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yogukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
◮Sisitemu yogukora neza ituma reaction igira sensibilité yo hejuru, gukomera kwumuriro, no kwihanganira neza.
◮RT-qPCR BiroroshyeTM.
Ibisobanuro
RT-qPCR BiroroshyeTM (Intambwe imwe) -SYBR Icyatsi I. ikoresha sisitemu idasanzwe kugirango ihuze neza RT na Real Time PCR reaction, irashobora kurangiza byihuse kumenya umubare wa gen.Igicuruzwa gifite kwihanganira gukomeye, umwihariko wo hejuru no gutuza.Igicuruzwa kirimo isosiyete idasanzwe ya Foregene HotStar Taq ADN Polymerase, ifite ibyiza byo kwihuta byihuse (2Kb / min), gukora neza cyane, ubushobozi bwihariye bwo kongera imbaraga, hamwe n’igipimo gito kidahuye ugereranije n’imisemburo isanzwe ya Taq.Ikoreshwa hano mugihe nyacyo RT-PCR kugirango igabanye amplification idasanzwe no kunoza ukuri kwa PCR.
Ibisobanuro
100 T (sisitemu ya 20μl)
500 T (sisitemu ya 20μl)
Ibigize ibikoresho
| RT-qPCR BiroroshyeTM(Intambwe imwe) -SYBR Icyatsi I. | ||
| Ibirimo | RT-02111 | RT-02112 |
| 100T (sisitemu ya 20μl) | 500T (sisitemu ya 20μl) | |
| 2 × RT-qPCR BiroroshyeTM Kuvanga-SYBR | 1ml | 1.7ml × 3 |
| 50 × ROX Yerekana irangi | 200μl | 1ml |
| Ubusa ddH2O | 1.7ml | 1.7ml × 3 |
| Igitabo gikubiyemo amabwiriza | Igice | Igice 1 |
Ibiranga ibyiza
.2O.
- Igikoresho kirashobora gusesengura byihuse kandi neza muburyo bwo gusesengura virusi ya RNA cyangwa gukurikirana RNA.
- Igikoresho gikoresha uburyo bwihariye bwa Foregene revers transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yo gukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
- Sisitemu yogukora neza ituma reaction igira ibyiyumvo byo hejuru byo gutahura, gukomera kwumuriro, no kwihanganira neza.
- RT-qPCR BiroroshyeTM.
Urupapuro rw'akazi
Imiterere yo kubika
1.Ubwikoreziimiterere
Inzira yose yubushyuhe bwo hasi yubusanduku bwo gutwara, kugirango umenye neza ko ibikoresho biri muri <4 ℃.
2.Ububiko imiterere
Igikoresho kibitswe kuri -20 ℃.Bika ibicuruzwa muri firigo ihoraho yubushyuhe kuri-20 ℃ ako kanya nyuma yo kwakirwa.Niba uburyo bwo kubika bukwiye, ibicuruzwantizitesha agaciro imikorere iyo ari yo yose mugihe cyumwaka 1.
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