RT-PCR Byoroshyeᵀᴹ I (Intambwe imwe)
Ibisobanuro:
◮Igikoresho kimwe cyintambwe ituma transcription transcription na PCR ikorerwa mumiyoboro imwe.Irakeneye gusa kongeramo inyandikorugero RNA, progaramu yihariye ya PCR na ddH yubusa ya RNase2O.
◮Isesengura-nyaryo ryuzuye rya RNA rirashobora gukorwa vuba kandi neza.
◮Igikoresho gikoresha uburyo bwihariye bwa Foregene reverse transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yogukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
◮Sisitemu yogukora neza ituma reaction igira sensibilité yo hejuru, gukomera kwumuriro, no kwihanganira neza.
Ibisobanuro
Foregene Intambwe imwe RT-PCR BiroroshyeTM Ibicuruzwa byuruhererekane byerekana reaction ihuriweho kuva RNA kugeza ADN ikubye kabiri, ni ukuvuga guhinduranya inyandiko hamwe no kongera PCR byuzuzwa muri reaction imwe ya centrifuge hamwe na sisitemu imwe ya reaction, yoroshya intambwe zigeragezwa, optimiztgahunda yo kugerageza, naItezimbere ubushakashatsi gukora neza.
RT-PCR BiroroshyeTM I(Intambwe imwe) ni intambwe imwe ya RT-PCR kit yakozwe byumwihariko na Foregene, itahura imikorere ikomeza kuva RT kugeza PCR mumiyoboro imwe.Igikorwa kiroroshye kandi cyihuse, gishobora kugabanya neza umwanda mugihe cyubushakashatsi.2×RT-PCR BiroroshyeTM Kuvanga bifite kwihanganira gukomeye, gutuza ubushyuhe, gukora neza kandi byihariyeity for amplification.
Ibigize ibikoresho
| RT-PCR BiroroshyeTMI (Intambwe imwe) | ||
| Ibirimo | RT-02011 | RT-02012 |
| 100T (sisitemu ya 20μl) | 500T (sisitemu ya 20μl) | |
| 2 × RT-PCR BiroroshyeTM Kuvanga | 1ml | 1.7ml × 3 |
| Ubusa ddH2O | 1.7ml | 1.7ml × 3 |
| Igitabo gikubiyemo amabwiriza | Igice 1 | Igice |
Ibiranga ibyiza
- Igikoresho kimwe cyintambwe ituma transcription transcription na PCR ikorerwa mumiyoboro imwe.Irakeneye gusa kongeramo inyandikorugero RNA, progaramu yihariye ya PCR na ddH yubusa ya RNase2O.
- Isesengura-nyaryo ryuzuye rya RNA rirashobora gukorwa vuba kandi neza.
- Igikoresho gikoresha uburyo bwihariye bwa Foregene revers transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yo gukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.
- Sisitemu yogukora neza ituma reaction igira ibyiyumvo byo hejuru byo gutahura, gukomera kwumuriro, no kwihanganira neza.
Urupapuro rw'akazi
Ububiko hamwe nubuzima bwa Shelf
1.Imiterere yo gutwara abantu
Inzira yose yubushyuhe bwo hasi yubushyuhe bwo gutwara, kugirango umenye neza ko ibikoresho biri muri <4 ° C.
2. Ububiko
Igikoresho kibitswe kuri -20 ° C.Bika ibicuruzwa muri firigo ihoraho yubushyuhe kuri -20 ° C ako kanya ukimara kuyakira.Niba uburyo bwo kubika bukwiye,tibicuruzwa ntabwo bizatesha agaciro imikorere iyo ari yo yose mugihe cyumwaka 1.
