Direct RT-qPCR Kit
Ibisobanuro:
◮ Direct RT-qPCR Kit, ukoresheje Foreasy Reverse Transcriptase na Foreasy HS Taq ADN Polymerase itezwa imbere na Foregene, hamwe na reaction idasanzwe, ituma iki gikoresho gifite imbaraga zikomeye kandi gihuza, kandi kirashobora kugerageza muburyo butaziguye ukoresheje sisitemu ya Foregene Lysis nkicyitegererezo.Iki gikoresho kirashobora gusaba COVID-19 nucleic aside detection hamwe nizindi bagiteri zitera indwara ya nucleic aside itahura ibikoresho.
Ibisobanuro
Igikoresho cya RT-qPCR gitanga ibikoresho bitandukanye bipfunyitse RT-qPCR hamwe na enzyme ikora nezaimvange (Taq 、 M-MLV 、 RI) ， bituma byoroha mugukora ibikoresho byinyuma-sisitemuGuhindura ， muburyo bworoshye bwo kugenzura.
Ibisobanuro
500T, 50.000T
Ibigize ibikoresho
| Ibirimo (20 μL sisitemu yo kwitwara) | IM-05111-01 | IM-05112-01 |
| 500 T. | 50.000 T. | |
| Uruvange rwa Enzyme | 500 μL× 1 | 50 mL× 1 |
| 2 × Direct RT-qPCR Buffer | 1 mL× 5 | 500 mL× 1 |
| Amabwiriza | 1 | 1 |
Intambwe Zikora
Mbere yo gutegura reagent, reagent zose ziri mubikoresho zigomba gukonjeshwa mubushyuhe bwicyumba hanyuma zikavangwa buhoro.
A: Itegure of Inyandikorugero na reagent
1. Tegura icyitegererezo cya RNA (Birasabwa gukoresha Foregene Yuzuye ya RNA Isolation Kit kit kugirango ikuremo kandi isukure icyitegererezo cya RNA) cyangwa ibicuruzwa byacitse (Birasabwa gukoresha sisitemu ya Foregene Lysis), primers yihariye (10 μM) nibindi bikoreshwa, ibikoresho.
2. Shyira 2 × Direct RT-qPCR Buffer, RNase-Yubusa ddH2O na 20 × Irangi ryerekana ROX (niba aribyo's bikenewe) mu gasanduku hamwe na barafu, bigatuma ibyo bishonga bisanzwe, kandi bivanze buhoro.
B: Itegure of RT-qPCR Sisitemu
Imbonerahamwe 1 : Itegure of RT-qPCR system
| Ibigize | Umubumbe | Kwibanda kwanyuma |
| 2 × Direct RT-qPCR Buffer | 10 μL | 1 × |
| Uruvange rwa Enzyme | 1μL | |
| Imbere Imbere (10 μM) | 0.8 μL | 50-900nM |
| Subiza Primer (10 μM) | 0.8 μL | 50-900nM |
| Ubushakashatsi (4 μM) | 1μL | 200nM |
| Inyandikorugero (RNA cyangwa Lysate) | X μL | |
| 20 × Irangi ryerekana irangi | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Umubare wuzuye | 20μL |
C: Shiraho i RT-qPCR reaction Porogaramu
Imbonerahamwe 2 : RT-qPCR reaction Porogaramu gushiraho (Kuri urugero: bibiri-intambwe buryo)
| Intambwe | Ubushyuhe | Igihe | Amagare | Ibirimo | |
| 1 | 50 ℃ | 15 min | 1* | 1 | Guhindura inyandiko |
| 2 | 95 ℃ | Imin | 1 | Mbere yo gutandukana | |
| 3 | 95 ℃ | Amasegonda 10 | 40-45 | Gutandukana | |
| 60 ℃ | 30 amasegonda 2 * | Umugereka / Kwagura | |||
Ububiko hamwe nubuzima bwa Shelf
-Igikoresho kigomba kubikwa kuri -20± 5℃.Igihe cyemewe ni amezi 12.
-Irinde gusubiramo inshuro nyinshi (<5 cycle).
