Direct RT-qPCR Kit II
Ibisobanuro:
◮Direct RT-qPCR Kit, ukoresheje Foreasy Reverse Transcriptase na Foreasy HS Taq ADN Polymerase itezwa imbere na Foregene, hamwe na reaction idasanzwe, ituma iki gikoresho gifite imbaraga zikomeye kandi gihuza, kandi kirashobora kugerageza muburyo butaziguye ukoresheje sisitemu ya Foregene Lysis nkicyitegererezo.Iki gikoresho kirashobora gusaba COVID-19 nucleic aside detection hamwe nizindi bagiteri zitera indwara ya nucleic aside itahura ibikoresho.
Ibisobanuro
Direct RT-qPCR Kit itanga ibikoresho bitandukanye bipfunyitse RT-qPCR, ikora neza cyane Foreasy HS Taq ADN Polymerasena Foreasy Reverse Transcriptase (MMLV),gukora byoroshye kurema ibikoresho-byanyuma nibikoresho bya sisitemu, muburyo bworoshye bwo kugenzura.
Direct RT-qPCR Kit, ukoresheje Foreasy Reverse Transcriptase na Foreasy HS Taq ADN Polymerase itezwa imbere na Foregene, hamwe na reaction idasanzwe, ituma iki gikoresho gifite imbaraga zikomeye kandi gihuza, kandi kirashobora kugerageza muburyo butaziguye ukoresheje sisitemu ya Foregene Lysis nkicyitegererezo.Iki gikoresho kirashobora gusaba COVID-19 nucleic aside detection hamwe nizindi bagiteri zitera indwara ya nucleic aside itahura ibikoresho.
Iki gikoresho gishobora kuba igice cyibicuruzwa bya IVD, ukoresheje byoroshye kandi byihuse.Irakeneye gusa kugenzura byoroshye, itongeye gutera imbere.
Ibisobanuro
500T, 50.000T
Ibigize ibikoresho
| Ibirimo (20 μL sisitemu yo kwitwara) | IM-05111-02 | IM-05112-02 |
| 500 T. | 50.000 T. | |
| Foreasy R.burigihe (200 U /μL) | 50 μL× 1 | 1 mL× 5 |
| Foreasy HS Taq ADN Polymerase (5 U /μL) | 100 μL× 1 | 1 mL× 10 |
| 2 × Direct RT-qPCRBuffer | 1 mL × 5 | 500 mL × 1 |
| Amabwiriza | 1 | 1 |
Intambwe Zikora
Igisubizo: Tegura inyandikorugero na reagent
1. Tegura icyitegererezo cya RNA (Birasabwa gukoresha Foregene Yuzuye ya RNA Isolation Kit kit kugirango ikuremo kandi isukure icyitegererezo cya RNA) cyangwa ibicuruzwa byacitse (Birasabwa gukoresha sisitemu ya Foregene Lysis), primers yihariye (10 μM) nibindi bikoreshwa, ibikoresho.
2. Shyira 2 × Directeur RT-qPCR Buffer, RNase-idafite ddH2O na 20 × ROX Reference Dye (niba bikenewe) mu isanduku irimo urubura, bigatuma ibyo bishonga bisanzwe, hanyuma bivangwa buhoro.
B: Tegura sisitemu ya RT-qPCR
Shaka kimwe cya kabiri cyijwi rya reaction ya reaction ya sisitemu (urugero, niba ingano ya sisitemu ari 20 μL, igomba kubona 10 μL 2 × Direct RT-qPCR Buffer). Kuriingano ya enzyme, turasaba koM-MLV ： 10-30 U / 20 μL sisitemu yo kwitwara,TaqADN polymerase：1-2 U / 20 μL sisitemu yo kwitwaraiki nicyo cyifuzo cyacu, ugomba kugerageza ukagihindura), ongeraho RNA inyandikorugero, primers yihariye na probe, hanyuma ongeraho RNase-Ubuntu ddH2O kugeza kuri 20 μL.Imyiteguro yihariye ya sisitemu ya reaction ya RT-qPCR irashobora koherezwa kumeza ikurikira.
Imbonerahamwe 1: Tegura sisitemu ya RT-qPCR
| Ibigize | Umubumbe | Kwibanda kwanyuma |
| 2 × Direct RT-qPCR Buffer | 10 μL | 1 × |
| Foreasy Reverse Transcriptase (MMLV) | 10-30 U. | |
| Foreasy HS Taq ADN Polymerase | 1-2 U. | |
| Imbere Imbere (10 μM) | 0.8 μL | 50-900nM |
| Subiza Primer (10 μM) | 0.8 μL | 50-900nM |
| Ubushakashatsi (4 μM) | 1 μL | 200nM |
| Inyandikorugero (RNA cyangwa Lysate) | X μL | |
| 20 × Irangi ryerekana irangi | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Umubare wuzuye | 20 μL |
Icyitonderwa: Imbere ya Primer na Reverse Primer ni primers yihariye ya gen.Sisitemu ya qPCR irashobora guhindurwa ukurikije urugero rwubushakashatsi na PCR.Turasaba 400nM kumurongo wanyuma wa primers nyinshi.Hindura ingano ya primers na probe ukurikije intumbero yateguwe hamwe nibisabwa byanyuma, nyamuneka.
1 *: Hitamo icyerekezo cyanyuma cya ROX Reference Dye ukurikije ibikoresho bitandukanye bya PCR.Ibyiza bya ROX Byerekana Irangi ryibikoresho bisanzwe bya PCR byerekanwe kumeza ikurikira:
| Ibikoresho bya PCR byuzuye | Iherezo rya ROX ryerekana irangi |
| ABI PRISM 7000/7300/7700/7900HT / Intambwe ya mbere,n'ibindi | 1 × (kurugero, ongeramo 1μl 20 × Irangi ryerekana ROX muri 20 μL sisitemu yo kwitwara) |
| ABI 7500, 7500 Byihuta, Stratagene Mx3000P, Mx3005P na Mx4000, nibindi. | 0.5 × (urugero, ongeramo 0.5μl 20 × Irangi ryerekana ROX muri sisitemu ya 20 μL) |
| Igikoresho cya Roche PCR, igikoresho cya Bio-Rad PCR, ibikoresho bya Eppendorf byuzuye PCR, nibindi. | Hatariho ROX Yerekana Irangi |
Ububiko hamwe nubuzima bwa Shelf
Igikoresho kigomba kubikwa kuri -20 ± 5 ℃.Igomba guhita ibikwa muri -20 fr firigo ya firimostatike.Igihe cyemewe ni imyaka 2 niba kibitswe muburyo bukwiye.
