Abatanga ibicuruzwa ninganda |Abakora ibicuruzwa mu Bushinwa

Icyerekezo Cyerekezo RT-qPCR Kit

◮Kuri RNA itaziguye-nyayo-nyayo kuva muri swab yakusanyije idafite inzira yo kweza RNA.Iki gikoresho cyuzuza qRT-PCR mugihe cyisaha imwe.Enzyme ivanze nuruvange rwiza rwa Reverse Transcriptase, Hot-Tangira Taq ADN polymerase, RNase inhibitor.Buffer ya Reaction ikubiyemo ibice byose bisabwa, harimo ibice byahinduwe neza, Mg²⁺, DUTP, na DNTP.

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FFPE ADN Yigunze Igikoresho cyo gukuramo ADN yo gukuramo ibikoresho bya FFPE

Byihuse usukure ADN yo mu rwego rwo hejuru ivuye mu bice byashyizwemo ibice nka para ﬃ n ibice na para ﬃ n bice.

◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.

◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.

◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.

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PCR Isukura Kit PCR Isukura Sisitemu ya Ultra-yera

Byihuse usukure kandi ubone ibice bya ADN byujuje ubuziranenge muri sisitemu ya PCR;niba ushaka gutandukana no kubona ibice bya ADN bya ADN, nyamuneka hitamo ibikoresho byo kugarura gel.

Range Urwego runini rwo kugarura ADN: Ibice bya ADN bigufi nka 60bp kandi binini nka 10kb birashobora kugarurwa.

Performance Gukora neza: Gukora neza muri rusange birenze 80%, kandi hejuru irashobora kugera hejuru ya 95%.

Speed Umuvuduko wihuse:Biroroshye gukora, kugarura ibice bya ADN birashobora kurangira muminota 15 kandi primer dimer irashobora gukurwaho bitabaye ngombwa gukira gel.

◮ S.umutekano: Nta gukuramo organic reagent bisabwa.

Quality Ubwiza bwo hejuru: Ibice bya ADN byagaruwe bifite isuku nyinshi, bishobora guhura nubushakashatsi butandukanye bwakurikiyeho.

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Icyitegererezo cyo Kurekura

◮Byoroshye gukora:icyitegererezo lysis mugihe cyo gukusanya icyitegererezo

AstByihuse kandi byoroshye:Ntibikenewe gukuramo aside nucleic, ibereye gutahura nini

ScopeUrwego rwagutse rwo gusaba:PCR mu buryo butaziguye nyuma yo gukusanya icyitegererezo

Umutekano kandi wizewe:Kora vuba vuba virusi, garanti umutekano kurwego runini

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RT-PCR Byoroshyeᵀᴹ I (Intambwe imwe)

◮Igikoresho kimwe cyintambwe ituma transcription transcription na PCR ikorerwa mumiyoboro imwe.Irakeneye gusa kongeramo inyandikorugero RNA, progaramu yihariye ya PCR na ddH yubusa ya RNase₂O.

◮Isesengura-nyaryo ryuzuye rya RNA rirashobora gukorwa vuba kandi neza.

◮Igikoresho gikoresha uburyo bwihariye bwa Foregene reverse transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yogukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.

◮Sisitemu yogukora neza ituma reaction igira sensibilité yo hejuru, gukomera kwumuriro, no kwihanganira neza.

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RT-PCR Byoroshyeᵀᴹ II (Intambwe ebyiri)

◮Isesengura-nyaryo ryuzuye rya RNA rirashobora gukorwa vuba kandi neza.

◮Igikoresho gikoresha uburyo bwihariye bwa Foregene reverse transcription reagent hamwe na Foregene HotStar Taq ADN Polymerase ihujwe na sisitemu idasanzwe yogukora kugirango tunoze neza imikorere ya amplification hamwe nibisobanuro bya reaction.

◮ Sisitemu yogukora neza ituma reaction igira sensibilité yo hejuru, gukomera kwumuriro, no kwihanganira neza.

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Imbeba Umurizo ADN Mini Kit Genomic ADN Ikuramo Ibikoresho biva murizo

Igikoresho cyihuta cyane kwisi gukuramo ADN ya genomic ADN, ishobora gukuramo ADN nziza cyane mumirizo yimbeba mugihe cyisaha 1.

◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.

◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.

◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.

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Bagiteri ya ADN Yigunze Kit Bakteri ya Genomic Gukuramo ibikoresho byo kweza

Byihuse usukure ADN ya genomic yo mu rwego rwo hejuru muri bagiteri mugice cyo gukura kwa logarithmic.

◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.

◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.

◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.

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CCND1 / IGH Amabara abiri Amabara abiri yo guhuza

Ukurikije ihame rya ADN yuzuzanya, ubushakashatsi bwa CCND1 orange-umutuku na IGH icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.

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RB1 / 1q21 Amabara abiri

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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HER2 / CSP17 Amabara abiri

Fluorescence muburyo bwa Hybridisation (FISH) ishingiye ku ihame ryo kuzuzanya gushingiye ku bice bya ADN, no kwerekana amashusho y’ibimenyetso bivangwa na fluorescence yanditseho ADN ya ADN hamwe na ADN ikurikirana muri selile nucleos munsi ya microscope ya fluorescence.

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ETV6 Ibice bibiri Kumena Ibice Bitandukanye

Dukurikije ihame fatizo ryuzuzanya rya ADN, iperereza rya ETV6 orange-umutuku na ETV6 icyatsi kibisi byakoreshejwe mu guhuza hamwe na ADN ikurikirana muri nucleus, kandi amakuru ya leta ya gene muri nucleus yarabonetse kandi arasesengurwa kuri microscope ya fluorescence.

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