Serumu (Plasma) miRNA Igikoresho cyo kwigunga
Ibisobanuro:
◆Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
◆Inkingi ya ADN-Isukura ihuza cyane na ADN, kugirango igikoresho gishobora kuvanaho ADN yanduye utongeyeho DNase.
◆Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
◆Umuvuduko wihuse: Igikorwa kiroroshye kandi kirashobora kurangira muminota 30.
◆Umutekano: Nta gukuramo ibinyabuzima bisabwa.
◆Ubwiza buhanitse: Ibice bya RNA byakuweho bifite isuku nyinshi, bitarimo poroteyine nibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.
Ibisobanuro
Iki gikoresho gikoresha spin inkingi hamwe na formula yakozwe na societe yacu, irashobora gukuramo neza uduce duto twa miRNA munsi ya 100nt muri serumu na plasma.Igikoresho gitanga ADN-Isukura Inkingi, ishobora gutandukanya byoroshye ndengakamere na tissue lysate na adsorb no gukuraho ADN murugero, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza neza RNA, kandi hamwe na formula idasanzwe, irashobora gukoreshwa mugihe kimwe.Tunganya umubare munini wintangarugero.
Sisitemu yose ni RNase-Yubusa, kugirango miRNA isukuye ntisuzugurwe;Buffer miBRW1 na Buffer miBRW2 sisitemu yo gukaraba ituma RNA ibona nta proteyine, ADN, ion, hamwe n’umwanda wanduye.
Ibisobanuro
5T, 50T, 100T, 200T
Ibigize ibikoresho
| Serumu / Plasma miRNA Igikoresho cyo kwigunga | ||||
| Ibirimo | RE-0111T | RE-01111 | RE-01112 | RE-01114 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miBRL1* | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRL2* | 1.2ml | 12ml | 24ml | 48ml |
| Buffer miBRW1 | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRW2 | 2.4ml | 24ml | 48ml | 96ml |
| Ubusa ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| Inkingi ya RNA gusa | 5 ibice | 50ibice | 100ibice | 200ibice |
| Inkingi ya ADN | 5 ibice | 50ibice | 100ibice | 200ibice |
| IFU | 1 igice | 1 igice | 1 igice | 1 igice |
Ibiranga & ibyiza
◆Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
◆Inkingi ya ADN-Isukura ihuza cyane na ADN, kugirango igikoresho gishobora kuvanaho ADN yanduye utongeyeho DNase.
◆Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
◆Umuvuduko wihuse: Igikorwa kiroroshye kandi kirashobora kurangira muminota 30.
◆Umutekano: Nta gukuramo ibinyabuzima bisabwa.
◆Ubwiza buhanitse: Ibice bya RNA byakuweho bifite isuku nyinshi, bitarimo poroteyine nibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.
Ibipimo by'ibikoresho
Porogaramu
Birakwiriye gukuramo no kweza miRNA mubikoko byinyamaswa na plasma byabantu.
miRNA
MiRNA yatunganijwe na Serum / Plasma miRNA Isolation Kit irashobora gukoreshwa mubushakashatsi butandukanye bwa molekile yo hepfo, nka: RT-PCR, Igihe nyacyo RT-PCR, Blot y'Amajyaruguru, gusesengura chip, nibindi.
Urujya n'uruza rw'akazi
Imiterere yo kubika
Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba(15-25 ° C).I.f ikeneye kubikwa igihe kirekire,nyamunekabibitswe kuri 2-8 ° C.
