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Ni irihe tandukaniro riri hagati ya PCR, RT-PCR, qPCR, na RT-qPCR
PCR nuburyo bukoreshwa mu kwagura ADN kuva ku gipimo gito cya ADN.RT-PCR ikoresha transcript yinyuma kugirango ikore ADN yerekana isoko ya RNA ishobora noneho kongerwa.PCR na RT-PCR mubisanzwe reaction yanyuma, mugihe qPCR na RT-qPCR bakoresha kinetics yikigereranyo cyibicuruzwa ...Soma byinshi -
Imbere yo kuvumbura ibiyobyabwenge: Kwibanda kuri RNA hamwe na molekile nto
Urukingo rwa mRNA rwa Pfizer kuri COVID rwongeye kwifuza gukoresha aside ribonucleic (RNA) nk'intego yo kuvura.Ariko, kwibasira RNA hamwe na molekile nto biragoye cyane.RNA ifite ibice bine byubaka gusa: adenine (A), cytosine (C), guanine (G), na uracil (U) isimbuza thimine ...Soma byinshi -
Imiti ya nucleic ni iki?
“Imiti ya acide nucleique” ikoresha “acide nucleic,” yerekeza ku bintu nka ADN na RNA bigenzura amakuru akomoka ku moko, nk'ibiyobyabwenge.Izi zituma kwibasira molekile nka mRNA na miRNA zidashobora kwibasirwa nibiyobyabwenge gakondo bifite uburemere buke buke hamwe nibiyobyabwenge bya antibody, kandi hariho g ...Soma byinshi -
Bamwe bafite uburambe kuri siRNA muri RT-PCR
1. Ubumenyi bwibanze (niba ushaka kubona igice cyubushakashatsi, nyamuneka wimure mu gice cya kabiri) Nkigisubizo gikomoka kuri PCR isanzwe, Igihe nyacyo PCR ikurikirana cyane cyane ihinduka ryibicuruzwa byongerewe imbaraga muri buri cyiciro cya amplification ya PCR reaction mugihe nyacyo thr ...Soma byinshi -
RT-PCR igeragezwa sisitemu yogutezimbere uburyo burambuye
.RNA yo mu rwego rwo hejuru igomba nibura kuba ndende-yuzuye kandi idafite inzitizi zinyuranye za transitase nka EDTA cyangwa SDS.Ubwiza bwa RNA bugena ntarengwa a ...Soma byinshi -
Byuzuye PCR primer igishushanyo nibisobanuro bya PCR
Igishushanyo mbonera (99% ibibazo birashobora gukemurwa) 1. Uburebure bwibanze: Igitabo gisaba 15-30bp, mubisanzwe nka 20bp.Imiterere nyayo nibyiza kuba 18-24bp kugirango tumenye neza, ariko igihe kirekire, primer ndende cyane nayo izagabanya umwihariko, kandi igabanye umusaruro.2. Prim ...Soma byinshi -
Kwirinda qRT-PCR yakozwe na Muganga mukuru bitonze
Umuntu wese avuga kubyerekeye ihame rya qRT-PCR igerageza, igishushanyo mbonera, gusobanura ibisubizo, nibindi, ariko ndatekereza ko ngomba gusangira nawe imikorere yubushakashatsi bwa qRT-PCR.Nibito, ariko bijyanye nibisubizo.Mbere yo gukora qRT-PCR, dukeneye kumva neza ibyacu ...Soma byinshi -
Kwirinda RT-qPCR
Igeragezwa rya RT-qPCR ririmo gukuramo RNA no gusuzuma ubuziranenge, guhinduranya inyandiko hamwe na qPCR intambwe eshatu, buri ntambwe ifite ingamba nyinshi zo kwirinda, tuzabimenyesha birambuye hepfo.Ⅰ.Isuzuma ryiza rya RNA Mubigeragezo bya RT-qPCR, nyuma yo kurangiza gukuramo RNA, ...Soma byinshi -
Intangiriro yuzuye mugihe nyacyo PCR
1. Gusobanukirwa kwambere Kuri iki cyiciro, dukeneye gusobanukirwa bimwe na terminologiya, kugirango twirinde gukora amakosa imbere ya bakuru bacu, nka: Ikibazo: Ni irihe tandukaniro riri hagati ya RT-PCR, qPCR, Igihe nyacyo PCR, nigihe nyacyo RT-PCR?Igisubizo: RT-PCR isubiza inyuma PCR (reba t ...Soma byinshi -
PCR, PCR nyinshi, Muburyo PCR, Hindura PCR, RT-PCR, qPCR （1） - PCR
PCR, PCR nyinshi, Muburyo PCR, Hindura PCR, RT-PCR, qPCR （1） - PCR Tuzatoranya ibitekerezo, intambwe nibisobanuro bya PCR zitandukanye Ⅰ.PCR Polymerase Chain Reaction, yitwa PCR, ni tekinoroji ya biologiya ikoreshwa muguhuza ibice bya ADN byihariye.Birashobora gufatwa nk ...Soma byinshi -
QRT-PCR ihame ryubushakashatsi
RT-qPCR yatejwe imbere nubuhanga busanzwe bwa PCR.Yongera imiti ya fluorescent (amarangi ya fluorescent cyangwa fluorescent probe) muri sisitemu gakondo ya PCR, kandi ikamenya inzira ya PCR yo kwagura no kwagura mugihe nyacyo ukurikije uburyo bwabo butandukanye bwa luminescent.Ikimenyetso cya Fluorescent ...Soma byinshi -
RT-PCR igeragezwa sisitemu yogutezimbere uburyo burambuye
Ⅰ.Ongera ibyiyumvo bya sisitemu yo kubyitwaramo: 1. Gutandukanya byinshi -bisanzwe RNA: Synthesis nziza ya cDNA ituruka kumurongo wo hejuru RNA.RNA-yuzuye cyane igomba kwemeza byibuze igihe kirekire kandi ntigizwe na inhibitor zidafite imisemburo yo gufata amajwi, nka EDTA cyangwa SDS.Ikirangantego ...Soma byinshi
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