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Escherichia Coli O157: H7 Igikoresho cyo Kumenya Acide Nucleic (PCR Fluorescent Probe Method / Lyophilisation)
Injangwe.FP103
Ikoreshwa mugushakisha byihuse no gusuzuma E. coli O157: H7 mubiribwa, ibiryo, ibyitegererezo byamazi hamwe nibidukikije.
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ADN ya virusi & RNA Isolation Kit ADN ya virusi na RNA yo gukuramo ibikoresho byo gutunganya ibikoresho
Injangwe.No.DR-01011 / 01012/01013
Kugirango usukure virusi ya ADN / RNA muri plasma, serumu, umubiri utagira selile, umubiri ndengakamere.
Gutandukanya vuba no kweza virusi ADN cyangwa RNA mubitegererezo nka plasma, serumu, amazi yumubiri utagira selile numuco ndengakamere.
Nta kwangirika kwa RNA.Ibikoresho byose ni RNase-Ubuntu
Byoroshye - ibikorwa byose birangirira ku bushyuhe bwicyumba
Byihuse - ibikorwa birashobora kurangira muminota 20
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA
Umutekano - nta reagent ikoreshwa
Ubushobozi bunini bwo gutunganya-bigera kuri 200μl byintangarugero birashobora gutunganywa buri gihe.
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Ibice by'inyamanswa ADN Yigunze Igikoresho cyo gukuramo ADN Ibikoresho by'inyamanswa
Gukuramo vuba no kweza ADN genomic biva ahantu henshi, nk'inyama z'inyamaswa, selile, nibindi.
◮Umuvuduko wihuse:ibikorwa byinshi kuruta protease isa kandi igogora ingero za tissue byihuse;
◮Biroroshye:birashobora kurangira muminota 50.
◮Byoroshye:Bikorewe ku bushyuhe bwicyumba.
◮Umutekano:nta reagent ngenga ikoreshwa.
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Amaraso ADN Mini Kit
Sukura vuba ADN ya genomic yo mu rwego rwo hejuru mumaraso ya anticagulée (<1ml).
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
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Mini Plasmid Mini Kit Rusange Plasmid Gukuramo ADN Miniprep Mini Kits
Byihuse usukure ADN nziza cyane ya plasmid ADN ya bagiteri yahinduwe kubushakashatsi bwibinyabuzima bisanzwe bya molekuline nko guhinduka no gusya kwa enzyme.
◮Kuraho RNA utongeyeho RNase
◮Byoroshye- centrifugation ikorwa mubushyuhe bwicyumba
◮Byihuse—Igikorwa gishobora kurangira mu minota 20
◮Umutekano—Nta reagent ikoreshwa
◮Isuku ryinshi—OD260 / 280≈1.7-1.9
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RT Byoroshyeᵀᴹ I Master Premix kumurongo wambere cDNA synthesis
- Sisitemu nziza yo kwandukura sisitemu, bifata iminota 25 gusa kugirango urangize synthesis yumurongo wambere cDNA.
- Sisitemu yo hejuru-yimikorere ya sisitemu yo kwandukura, pg-urwego rwicyitegererezo na rwo rushobora kubona cDNA nziza.
- Sisitemu yo kwandukura sisitemu ifite ubushyuhe buhanitse, ubushyuhe bwa reaction ya sisitemu irashobora kuba hejuru ya 50 ℃,kandi ifite imikorere myiza yo guhindura inyandiko.
- Ndetse na RNA yanduye (inzoga zigera kuri 45%, umunyu wa guanidine kugeza kuri 750mM) urashobora gukorerwa reaction ya reaction.
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RT Byoroshye II (hamwe na gDNase) Master Premix kumurongo wa mbere wa cDNA synthesis ya Real Time PCR hamwe na gDNase
-Ubushobozi buhagije bwo gukuraho gDNA, ishobora gukuraho gDNA mubishusho muminota 2.
-Uburyo bwiza bwo kwandukura sisitemu, bifata iminota 15 gusa kugirango urangize synthesis yumurongo wambere cDNA.
-Icyitegererezo cyoroshye: inyandikorugero zirimo ibintu byinshi bya GC hamwe nuburyo bugoye bwa kabiri birashobora kandi guhindurwa hamwe nubushobozi buhanitse.
-Ibihe byinshi-byunvikana sisitemu yo kwandukura, pg-urwego rwicyitegererezo nacyo gishobora kubona cDNA nziza.
-Uburyo bwo kwandukura sisitemu ifite ubushyuhe bwinshi, ubushyuhe bwiza ni 42℃, kandi iracyafite imikorere myiza yo kwandukura kuri 50℃.
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Igihe nyacyo PCR Byoroshyeᵀᴹ-SYBR Icyatsi I kit
Byoroshye-2X PCR Kuvanga kugirango ugabanye ikosa ryikigereranyo nigihe cyo gukora
Byihariye-byateguwe neza na hot-itangira Taq enzyme irashobora gukumira amplification idasanzwe hamwe na primer dimer
Ubukangurambaga bukabije - bushobora kumenya kopi ntoya yicyitegererezo
Guhindura byinshi-bihujwe nibikoresho nyabyo-byuzuye bya PCR
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Amatungo Yuzuye RNA Yigunga Igikoresho Cyuzuye RNA Gukuramo no Kweza Ibikoresho Byinyamanswa & Akagari
Byihuse kandi neza gukuramo RNA-yuzuye kandi yuzuye-yuzuye muri RNA inyama zinyamaswa zitandukanye.
Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA.Sisitemu yose ni RNase-Yubusa
Kuraho neza ADN ukoresheje Inkingi-Yeza
Kuraho ADN utongeyeho DNase
Byoroshye - ibikorwa byose birangirira ku bushyuhe bwicyumba
Byihuse - ibikorwa birashobora kurangira muminota 30
Umutekano - nta reagent ikoreshwa
Isuku ryinshi - OD260 / 280≈1.8-2.1
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Akagari Igiteranyo Cyuzuye RNA Igikoresho Cyuzuye RNA Yigunga Ibikoresho byo muri selire
RNA isukuye cyane kandi yujuje ubuziranenge RNA irashobora kuboneka mumasoko atandukanye yimico muminota 11.
RNase-Ubuntu
Bikorera mubushyuhe bwicyumba (15-25 ℃)
Hamwe na ADN-Isukura Inkingi
Umusaruro mwinshi wa RNA
Byihuse: Kurangiza gukuramo muminota 11
Umutekano: Nta miti kama
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Amaraso ADN Midi Kit (1-5ml) Gutandukanya Amaraso Midi Ibikoresho bya ADN biva mumaraso
Sukura vuba ADN ya genomic yo mu rwego rwo hejuru mumaraso ya anticagulée (1-5ml).
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
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Buccal Swab / Ikarita ya FTA Ikarita ya ADN Yigunga Kit Genomic ADN Gukuramo cyangwa Igikoresho cyo Kweza muri Buccal Swabs
Sukura vuba ADN ya genomic yo mu rwego rwo hejuru ya buccal swab / Ikarita ya FTA.
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
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