Gutera Igiteranyo Cyuzuye RNA Kwigunga
Ibisobanuro:
Kuraho neza ADN ukoresheje Inkingi-yoza
Kuraho ADN utongeyeho DNase
Byoroshye - ibikorwa byose birangirira mubushyuhe bwicyumba
Byihuse - ibikorwa birashobora kurangira muminota 30
Umutekano - nta reagent ikoreshwa
Ibisobanuro
50 Imyiteguro, 200 Imyiteguro
Igikoresho gikoresha spin inkingi hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nziza kandi yuzuye yuzuye ya RNA mubice bitandukanye byibimera bifite polysaccharide cyangwa polifenole nyinshi.Itanga ADN-Isukura inkingi ishobora gukuramo ADN genomic muri supernatant na tissue lysate.Inkingi ya RNA yonyine irashobora guhuza RNA neza.Igikoresho kirashobora gutunganya umubare munini wicyitegererezo icyarimwe.
Sisitemu yose ntabwo irimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer PRW1 na Buffer PRW2 birashobora kwemeza ko RNA yabonye itanduye na poroteyine, ADN, ion, hamwe n’ibinyabuzima.
Ibigize ibikoresho
| Buffer PSL1, Buffer PS, Buffer PSL2 |
| Buffer PRW1, Buffer PRW2 |
| Ubusa ddH2O, ADN-Isukura Inkingi |
| RNA-Inkingi gusa |
Ibiranga & ibyiza
Gukorera mubushyuhe bwicyumba (15-25 ℃) mubikorwa byose, nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
■ By'umwihariko bikwiriye kwezwa na RNA kubihingwa bya polysaccharide na polifenol.
Inkingi ya ADN-Isukura Inkingi ihuza cyane na ADN, kugirango ibikoresho bishobore gukuraho ADN yanduye utongeyeho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa na formula idasanzwe irashobora kweza neza RNA.
Speed Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 30.
Umutekano: nta reagent ikenewe.
Quality Ubwiza buhanitse: Ibice bya RNA bisukuye bifite isuku nyinshi, bitarimo poroteyine n’ibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.
Ibipimo byibicuruzwa
Applications Porogaramu yo hasi: synthèse ya mbere ya cDNA, RT-PCR, clone ya molekulari, Amajyaruguru, nibindi.
Ample Icyitegererezo: Ibimera bishya cyangwa bikonje bya polysaccharide na polifenol
Ingano: 50mg ingirangingo
Ubushobozi ntarengwa bwa RNA bwo guhuza inkingi: 80 μg
Volume Ingano ya Elution: 50-200 μl
Porogaramu
Birakwiriye gukuramo no kweza RNA yose uhereye kumyumbati y'ibiti bishya cyangwa bikonje (cyane cyane ibibabi byibiti bishya) hamwe na polysaccharide nyinshi hamwe na polifenol.
Urujya n'uruza rw'akazi
Igishushanyo
Gutera Igiteranyo cya RNA Isolation Kit Plus yatunganije 50mg yamababi mashya ya polysaccharide na polifenol, naho 5% ya RNA yapimwe na electrophorei.
1: Umuneke
2: Ginkgo
3: Impamba
4: Amakomamanga
Ububiko hamwe nubuzima bwa Shelf
Iki gikoresho gishobora kubikwa amezi 24 mugihe cyumutse mubushyuhe bwicyumba (15-25 ℃);niba ikeneye kubikwa igihe kirekire, irashobora kubikwa muri 2-8 ℃.
Buffer PSL1 irashobora gushirwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc-mercaptoethanol (birasabwa kongeramo mugihe kimwe cyo kugerageza).
