Urutonde rwa ADN yo kwigunga Abatanga Uruganda |Ubushinwa Urukurikirane rwa ADN rwitandukanya Uruganda

PCR Isukura Kit PCR Isukura Sisitemu ya Ultra-yera

Byihuse usukure kandi ubone ibice bya ADN byujuje ubuziranenge muri sisitemu ya PCR;niba ushaka gutandukana no kubona ibice bya ADN bya ADN, nyamuneka hitamo ibikoresho byo kugarura gel.

Range Urwego runini rwo kugarura ADN: Ibice bya ADN bigufi nka 60bp kandi binini nka 10kb birashobora kugarurwa.

Performance Gukora neza: Gukora neza muri rusange birenze 80%, kandi hejuru irashobora kugera hejuru ya 95%.

Speed Umuvuduko wihuse:Biroroshye gukora, kugarura ibice bya ADN birashobora kurangira muminota 15 kandi primer dimer irashobora gukurwaho bitabaye ngombwa gukira gel.

◮ S.umutekano: Nta gukuramo organic reagent bisabwa.

Quality Ubwiza bwo hejuru: Ibice bya ADN byagaruwe bifite isuku nyinshi, bishobora guhura nubushakashatsi butandukanye bwakurikiyeho.

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Bagiteri ya ADN Yigunze Kit Bakteri ya Genomic Gukuramo ibikoresho byo kweza

Byihuse usukure ADN ya genomic yo mu rwego rwo hejuru muri bagiteri mugice cyo gukura kwa logarithmic.

◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.

◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.

◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.

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Imbeba Umurizo ADN Mini Kit Genomic ADN Ikuramo Ibikoresho biva murizo

Igikoresho cyihuta cyane kwisi gukuramo ADN ya genomic ADN, ishobora gukuramo ADN nziza cyane mumirizo yimbeba mugihe cyisaha 1.

◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.

◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.

◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.

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Igikoresho cyo gukuramo Gel ADN Ikuramo ibikoresho

Byihuse kandi e ﬃ byoroshye kugarura ibice 20bp-10kb bya ADN biva muri gel ya agarose.

◮Umubare munini wo gukira ADN:Ibice bya ADN bigufi nka 30bp kandi binini nka 10kb birashobora kugarurwa.

◮Uburyo bwiza bwo gukira:Uburyo bwiza bwo gukira bushobora kugera hejuru ya 80%.

◮Sisitemu ntoya:byibuze igisubizo cya 30μl gishobora gukoreshwa mugukuraho, gishobora kongera neza kwibumbira hamwe kwa ADN yagaruwe.

◮Umuvuduko wihuse:Byoroshye gukora, kugarura ibice bya ADN birashobora kurangira muminota 15.

◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.

◮Ubwiza bwo hejuru:Ibice bya ADN byagaruwe bifite isuku nyinshi, bishobora guhura nubushakashatsi butandukanye bwakurikiyeho.

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EndoFree Maxi Plasmid Kit (Inkingi izunguruka)

Ibikorwa byose byo gukuramo bifata isaha 1 gusa, byemeza imikorere yoroshye kandi yihuse.

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