Niki PCR itaziguye

PCR itaziguye nuburyo bwo kongera ADN kuvamaraso, inyama zinyamaswa,umurizo,selire, ugutwi kw'imbeba,zebra amafi, amagi y'amafi,amababi y'ibihingwa,imbutocyangwa ubundi buryo bwa biologiya bwumwimerere ntagukora ADN yo kwigunga no kweza.Tekinike ya PCR itaziguye irashobora kugabanya cyane igihe cyubushakashatsi, nigiciro muri genotyping hamwe nimishinga myinshi.Itanga kandi amahitamo meza mugihe uhuye ningorane zo kongera umubare muto wicyitegererezo aho intambwe yo kweza ishobora gutera igihombo cyicyitegererezo.

PCR ya PCR ya FOREGENE ifite imbaraga zo guhangana n’ingutu, kubera imbaraga za FOREGENE zatanzwe na Taq enzyme (ipatanti yemewe: ZL20161034512.0 (Ubushinwa), EP3450560 (Uburayi), PCT / CN2017 / 082154 (Amerika), ipatanti 6894926 (Ubuyapani)).Enzyme ya Taq ikoresha ikorana buhanga rya ADN kugirango yongere guhuza ADN ihuza akarere ka acide nucleic aside ihuza poroteyine hamwe na polymerase ya Taq irwanya ubushyuhe kugirango yubake ADN yerekana urugero rwinshi.Gutezimbere gushyushye-gutangira Taq igumana imirimo itandukanye ya polymerase yumwimerere ya ADN.Iyi misemburo ya Taq irashobora guhuza na ADN yerekana icyitegererezo hanyuma igatangiza synthesis ya ADN mubishusho bito hamwe nibidukikije bigoye.

For urugero,itni byoroshye nko kongeramo ibyaweselire,igihingwa (ikibabi gito, umuzi cyangwa imbuto) cyangwa icyitegererezo cyinyamanswa kuribuffer, no kongeramo umwiharikoshobora kuvanga nayihariyeprimers muri tebes ya PCR no kuyinyuza mumagare yumuriro.Ibikoresho byiza nibyiza kubisesengura binini byerekana urugero aho kubika umwanya ari ngombwa cyane.

Sisitemu ya reaction ya PCR irimo enzyme ya UNG na dUTP yatoranijwe ukurikije ibisabwa mubushakashatsi, ishobora kwirinda neza umwanda uterwa nibicuruzwa byongera PCR.