Lnc-RT Intwariᵀᴹ I (Hamwe na gDNase) (Super Premix ya synthesis ya cDNA ya mbere kuva lncRNA)
Ibisobanuro
25×20μl Rxns, 50×20μRxns
Lnc-RT IntwariTM I (Hamwe na gDNase) ni sisitemu yo kwandukura ibintu byakozwe muburyo bwihariye kuri lncRNA kugirango ikureho vuba ADN yanduye.5 × gDNase Ivanga irashobora gukuraho vuba genome isigaye muri RNA kuri 42 ° C muminota 2, birinda neza kwivanga kwa genome kubisubizo bya qPCR.
5 × L-RT IntwariTM ivanze irimo Foregene LncRNA Reverse Transcriptase yakozwe na Foregene, ni ubwoko bushya bwa transcript transcriptase cyane cyane kuri lncRNA hamwe nizindi ndende ndende za RNA, hamwe na RNA ikomeye kandi ikora neza.Sisitemu itezimbere ituma igipimo cyo kwandukura cyihuta kandi gishobora kwandukura byoroshye inyandikorugero ya RNA hamwe nibintu byinshi bya GC hamwe nuburyo bugoye bwa kabiri.Synthesis ya mbere ya cDNA irashobora kurangira kuri 42 ° C muminota 15.
Ibigize ibikoresho
| 5× gDNase Kuvanga |
| 5× L-RT Intwari |
| RNase Yubusa ddH2O |
| Amabwiriza |
Ibiranga ibyiza
■Ubushobozi buhagije bwo gukuraho gDNA, ishobora gukuraho gDNA mubishusho muminota 2.
Can Irashobora guhindura neza transcript ya lncRNA.Iyo ibicuruzwa biva mu mahanga byatewe na qPCR, agaciro ka Ct kari munsi yubwa sisitemu isanzwe yo kwandukura.
System Uburyo bwiza bwo guhindura inyandiko, bifata iminota 15 gusa kugirango urangize synthesis yumurongo wambere cDNA.
Templates Inyandikorugero zigoye: inyandikorugero zirimo GC ndende hamwe nuburyo bugoye bwa kabiri birashobora kandi guhindurwa hamwe nubushobozi buhanitse.
■ Kwiyunvikana cyane sisitemu yo kwandukura sisitemu, pg-urwego rwicyitegererezo nacyo gishobora kubona cDNA nziza.
Sisitemu yo kwandukura sisitemu ifite ubushyuhe bwinshi, ubushyuhe bwiza ni 42℃, kandi iracyafite imikorere myiza yo kwandukura kuri 50℃.
Porogaramu
lncRNA isesengura ryinshi.
lncRNA isesengura ryuzuye.
Irashobora gusesengura byihuse kandi neza inzira ya RNA nka virusi ya RNA.
Guhindura inyandiko mvugo ya RNA ifite ibintu byinshi bya GC cyangwa urwego rwisumbuyeho.
Urujya n'uruza rw'akazi
Ububiko hamwe nubuzima bwa Shelf
Igikoresho kigomba kubikwa kuri -20°C. Bika ibicuruzwa muri firigo ihoraho yubushyuhe kuri -20°C ako kanya nyuma yo kwakirwa.Niba uburyo bwo kubika bukwiye, ibicuruzwa ntibishobora gutesha agaciro imikorere iyo ari yo yose mugihe cyumwaka 1.
Igitabo cyo gusesengura ibibazo
The following is an analysis of the problems that may be encountered in the use of Lnc-RT HeroTM I (With gDNase) series kits, hoping to be helpful to your experiments. In addition, we have dedicated technical support to help you with other experimental or technical problems beyond the operating instructions and questions. If you have any needs, please contact us: 028-83361257 or E-mail: Tech@foregene.com.
Non-amplification yihariye
1. Igishushanyo mbonera kidafite ishingiro
Icyifuzo: Shushanya primers ukurikije amahame yo gushushanya.
2. Ibisigisigi bya genome.
Igitekerezo: Menya neza ko intambwe yambere yo gukuraho ubushyuhe bwa gDNA ari 42 ° C, kandi igihe cyo kubyitwaramo nacyo gishobora kwongerwa kugeza 5min.
Nta kimenyetso cyo kongera imbaraga na RT-qPCR
1. RNA yangiritse.
Icyifuzo: Ibikoresho byo gukuramo RNA bigomba kuba bishya bishoboka, kandi hagomba gukoreshwa RNA yo mu rwego rwo hejuru kandi yera cyane.
2. RNA irimo inhibitor.
Icyifuzo: Inhibitori ya transcription yisubiramo muri rusange harimo SDS, umunyu wa guanidine, EDTA, nibindi. Birasabwa koza imvura ya RNA hamwe na 70% Ethanol kugirango ikureho inhibitor.
3. Ibibazo byo gushushanya.
Icyifuzo: Ukurikije ihame ryibishushanyo mbonera, ongera utegure primers kugirango ugenzurwe.
Intego yintego igaragara mugucunga ubusa
1. Kwanduza ibikoresho bikora cyangwa reagent.
Icyifuzo: Reagent cyangwa ibikoresho byose byo kugerageza bigomba kuba autoclave.Witondere kandi witonda mugihe ukora kugirango wirinde ingero za ADN zinjizwa muri pipette cyangwa zisuka mu muyoboro wa centrifuge.
2. Umwanda wabaye mugihe cyo gutegura sisitemu ya reaction ya PCR.
Icyifuzo: Fata ingamba zikenewe mugihe ukemura, nka: kwambara uturindantoki twa latex, ukoresheje inama zo kuyungurura.Koresha Igihe nyacyo PCR Ivange muri sisitemu-yanduye.
3. Intangiriro zateshejwe agaciro.
Igitekerezo: Koresha amashanyarazi ya SDS-PAGE kugirango umenye niba primers yangiritse, hanyuma usimbuze primers nshya kubushakashatsi bwa fluorescence.
Igitabo gikubiyemo amabwiriza:
Lnc-RT Intwari I (Hamwe na gDNase)
