Twibwira ko abakiriya batekereza, byihutirwa gukora bivuye ku nyungu zumuguzi wihame, kwemerera ubuziranenge bwo hejuru, kugabanya ibiciro byo gutunganya, igiciro cyibiciro birumvikana cyane, gutsindira ibyifuzo bishya kandi bishaje gushyigikirwa no kwemeza kugurishwa kwinshi mubushinwa Rna Nucleic Acide Extraction / Isukura Reagent hamwe na Virus Sampling Swab Kit (Uburyo bwa Magnetique) hamwe na CE na ISO, Tugiye kongera kugerageza gufasha abaguzi bo mu gihugu ndetse n’amahanga, kandi tubyare inyungu n’ubufatanye hagati yacu.dutegereje cyane ubufatanye bwawe buvuye ku mutima.
Twibwira ko abakiriya batekereza, byihutirwa gukora bivuye ku nyungu zumwanya wumuguzi wihame, kwemerera ubuziranenge bwo hejuru, kugabanya ibiciro byo gutunganya, ibiciro birashyirwa mu gaciro, gutsindira ibyifuzo bishya kandi bishaje inkunga no kwemezwa kuriUbushinwa Gukuramo Acide Nucleic Reagent, Uburyo bwa Magnetique, Twiyubashye nka sosiyete igizwe nitsinda rikomeye ryinzobere zifite udushya kandi inararibonye mubucuruzi mpuzamahanga, guteza imbere ubucuruzi no guteza imbere ibicuruzwa.Byongeye kandi, isosiyete ikomeza kuba umwihariko mubanywanyi bayo bitewe nubuziranenge bwayo buhebuje mu musaruro, kandi ikora neza kandi ihinduka mu gutera inkunga ubucuruzi.

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Twibwira ko abakiriya batekereza, byihutirwa gukora bivuye mubyifuzo byumuguzi wihame, kwemerera ubuziranenge bwo hejuru, kugabanya ibiciro byo gutunganya, igiciro cyibiciro birumvikana cyane, gutsindira ibyifuzo bishya kandi bishaje gushyigikirwa no kwemezwa kugurishwa kwinshi mubushinwa Rna Nucleic Acide Gukuramo / Kweza Reagent hamwe no gutsindira inyungu hamwe no guharanira inyungu hamwe no kugura inyungu hamwe no kugura inyungu hamwe na hamwe na hamwe na virusi ya Sampling Swab Kit (Magnetic Bead Metho) ubufatanye hagati yacu.dutegereje cyane ubufatanye bwawe buvuye ku mutima.
Kugabanuka kwinshiUbushinwa Gukuramo Acide Nucleic ReagentUburyo bwa Magnetic Bead Method, Twiyubashye nkisosiyete igizwe nitsinda rikomeye ryinzobere zifite udushya kandi inararibonye mubucuruzi mpuzamahanga, guteza imbere ubucuruzi no guteza imbere ibicuruzwa.Byongeye kandi, isosiyete ikomeza kuba umwihariko mubanywanyi bayo bitewe nubuziranenge bwayo buhebuje mu musaruro, kandi ikora neza kandi ihinduka mu gutera inkunga ubucuruzi.