Ubushinwa Ubushinwa Bwinshi Ubushinwa Ibihe Byose Byiza Byiza Igiciro-Cyiza PCR Ihingura nuwitanga |Foregene

Ubushinwa Bugurisha Ubushinwa Ibihe Byose Byiza Byiza-Bikora PCR

Ubushinwa Bwinshi Ubushinwa Ibihe Byose Byiza Byiza Igiciro-Cyiza PCR Ikiranga Ishusho

Ibisobanuro:

Iki gikoresho gikoresha sisitemu yihariye ya lysis kugirango irekure vuba ADN genomic muri zebrafish nizindi nyama zamafi yamazi meza, imirizo yumurizo cyangwa amagi y amafi byerekana PCR, bityo rero birakwiriye cyane cyane kwipimisha geneti nini.Igikorwa cyo kurekura ADN genomic muri buffer ya lysis kirangiye muminota 10-30 kuri 65 ° C.Ntayindi nzira nka protein na RNA ikuraho, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2 × PCR EasyTM Ivanga ifite kwihanganira bikomeye PCR ibuza gukora, kandi irashobora gukoresha lysate yicyitegererezo kugirango igeragezwe nkicyitegererezo cyo gukora neza kandi cyihariye.Iyi reagent irimo ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, buffer reaction, optimizer ya PCR na stabilisateur.Ikoreshwa ifatanije na lysis buffer irashobora kubona byihuse kandi byoroshye ingero, kandi ifite ibiranga sensibilité yo hejuru, umwihariko ukomeye, hamwe no guhagarara neza.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Urufunguzo rwo gutsinda kwacu ni "Ubwiza bwibicuruzwa byiza, Agaciro gafatika na serivisi nziza" kubushinwa Bwinshi Ubushinwa Ibihe Byose Ibihe Byiza-Bikoresha neza PCR, "Ubwiza", "ubunyangamugayo" na "serivisi" nihame ryacu.Ubudahemuka n'ibyo twiyemeje bikomeza kubahwa kubikorwa byawe.Vugana natwe uyumunsi Kubindi bisobanuro, kora natwe nonaha.
Urufunguzo rwo gutsinda kwacu ni "Ibicuruzwa byiza byiza, Agaciro gafatika na serivisi nziza" kuriPCR nziza, Ubushinwa Igihe cyose PCR, Ikipe yacu izi neza ibyifuzo byisoko mubihugu bitandukanye, kandi irashoboye gutanga ibintu byiza bikwiye kubiciro byiza kumasoko atandukanye.Isosiyete yacu yamaze gushyiraho itsinda ryujuje ibyangombwa, rihanga kandi rifite inshingano zo guteza imbere abakiriya bafite ihame-ryinshi.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Buffer FP
	Foregene Protease
	6 × ADN Yipakurura Buffer
Igice cya II	2 × PCR Biroroshye^TMKuvanga
Amabwiriza

Ibiranga ibyiza

■ Nta bisabwa bitwara igihe kandi bihenze bya ADN bisabwa.

Require Icyitegererezo gikenewe ni gito, nkibishobora 1mg amafi ya caudal fin cyangwa amagi 10 y amafi arashobora gupimwa.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ibipimo by'ibikoresho

Gushyira mu bikorwa: Amafi ya Zebra nandi mafi yamazi meza.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Urujya n'uruza rw'akazi

wk-flw-zebra-ifi-itaziguye-PCR

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer FP reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

v Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

vReagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

vReagent 2 × PCR EasyTM ivanze, niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ kubikwa igihe gito (koresha muminsi 10).

Urufunguzo rwo gutsinda kwacu ni "Ibicuruzwa byiza, Agaciro gafatika na serivisi nziza" kubushinwa Bwinshi Ubushinwa Ibihe Byose Ibihe Byiza Byiza-Gukoresha PCR 185 / 70r13, "Ubwiza", "ubunyangamugayo" na "serivisi" nihame ryacu.Ubudahemuka n'ibyo twiyemeje bikomeza kubahwa kubikorwa byawe.Vugana natwe uyumunsi Kubindi bisobanuro, kora natwe nonaha.
Ubushinwa Bwinshi Mubushinwa Igihe Cyose PCR, Ubwiza Bwiza PCR, Ikipe yacu izi neza isoko ryamasoko mubihugu bitandukanye, kandi irashobora gutanga ibicuruzwa byiza bikwiye kubiciro byiza kumasoko atandukanye.Isosiyete yacu yamaze gushyiraho itsinda ryujuje ibyangombwa, rihanga kandi rifite inshingano zo guteza imbere abakiriya bafite ihame-ryinshi.

Mbere: Uruganda rutanga Ubushinwa Rna ADN Nucleic Acide Gukuramo Reagent

Ibikurikira: Igishushanyo Cyamamare Mubushinwa Ikoreshwa rya virusi Sampling Tube hamwe na Swabs kubikoresho byo kwipimisha

Andika ubutumwa bwawe hano hanyuma utwohereze