Dukurikiza amahame yubuyobozi bwa "Ubwiza ni budasanzwe, Ubufasha ni bwo buhebuje, Icyubahiro ni icyambere", kandi tuzashiraho tubikuye ku mutima kandi dusangire intsinzi hamwe n’abakiriya bose kuri interineti yohereza ibicuruzwa mu mahanga Ubushinwa CE Yemeje Acide Nucleic Acide 48 Iriba rya plaque ADN / Rna Gukuramo Isolation Reagent Kit kubizamini bya PCR, Kubindi bisobanuro, nyamuneka twohereze imeri.Turimo kureba imbere amahirwe yo kugukorera.
Dukurikiza amahame yubuyobozi bwa "Ubwiza ni budasanzwe, Imfashanyo irarenze, Icyubahiro nicyambere", kandi tuzashiraho tubikuye ku mutima kandi dusangire intsinzi nabakiriya bose kuriUbushinwa Nucleic Acide Isolation Kit, Virus Nucleic Acide Isolation, Murakaza neza gusura isosiyete yacu, uruganda nicyumba cyacu cyerekana ibicuruzwa bitandukanye bizahuza nibyo witeze.Hagati aho, biroroshye gusura urubuga rwacu, kandi abakozi bacu bagurisha bazagerageza uko bashoboye kugirango baguhe serivise nziza.Menya neza ko utwandikira niba ukeneye amakuru menshi.Intego yacu ni ugufasha abakiriya kumenya intego zabo.Turimo gukora ibishoboka byose kugirango iki kibazo cyunguke.

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Dukurikiza amahame yubuyobozi bwa "Ubwiza ni budasanzwe, Ubufasha ni bwo buhebuje, Icyubahiro ni icyambere", kandi tuzashiraho tubikuye ku mutima kandi dusangire intsinzi hamwe n’abakiriya bose kuri interineti yohereza ibicuruzwa mu mahanga Ubushinwa CE Yemeje Acide Nucleic Acide 48 Iriba rya plaque ADN / Rna Gukuramo Isolation Reagent Kit kubizamini bya PCR, Kubindi bisobanuro, nyamuneka twohereze imeri.Turimo kureba imbere amahirwe yo kugukorera.
Abashoramari kumurongoUbushinwa Nucleic Acide Isolation Kit, Virus Nucleic Acide Isolation, Murakaza neza gusura isosiyete yacu, uruganda nicyumba cyacu cyerekana ibicuruzwa bitandukanye bizahuza nibyo witeze.Hagati aho, biroroshye gusura urubuga rwacu, kandi abakozi bacu bagurisha bazagerageza uko bashoboye kugirango baguhe serivise nziza.Menya neza ko utwandikira niba ukeneye amakuru menshi.Intego yacu ni ugufasha abakiriya kumenya intego zabo.Turimo gukora ibishoboka byose kugirango iki kibazo cyunguke.