Ubushinwa Abashinwa babigize umwuga Bikora cyane kandi bihamye birebire bya Taq ADN Polymerase hamwe na Loading Buffer hamwe na DNTP PCR Igikoresho nogukora |Foregene

Abashinwa babigize umwuga bakora neza kandi bihamye Taq ADN Polymerase hamwe na Loading Buffer na DNTP PCR Kit

Abashinwa babigize umwuga Bikora neza kandi bihamye Taq ADN Polymerase hamwe na Loading Buffer na DNTP PCR Kit Ikiranga Ishusho

Ibisobanuro:

Koresha mu buryo butaziguye imbuto ya polysaccharide polifenol lysate nkicyitegererezo cyo kongera PCR, udakuyemo ADN zitandukanye, byihuse kandi byoroshye.Gahunda ya UNG yo kurwanya umwanda yongeweho kugirango ikumire ibyiza bitari byo.

- Nta gutwara igihe kandi bihenze byo kweza ADN.

- Icyitegererezo gisabwa ni gito, fata imbuto imwe.

- Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

- Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

- Sisitemu yo kurwanya umwanda PCR 2 × Imbuto PCR EasyTM ivanze (UNG), ishobora gukuraho neza umwanda uterwa n’ibicuruzwa bya PCR kandi ikemeza umwihariko n’ukuri kwa amplification.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Iterambere ryacu riterwa nibicuruzwa bisumba byose, impano zikomeye kandi byongerewe imbaraga imbaraga zikoranabuhanga kubashinwa babigize umwuga bakora neza kandi bihamye kandi birebire birebire bya Taq DNA Polymerase hamwe na Loading Buffer na DNTP PCR Kit, Hamwe n’ubuyobozi bw’inganda, ubucuruzi buri gihe bwiyemeje gushyigikira ibyifuzo byo kuzaba umuyobozi w’isoko muri iki gihe mu nganda zabo.
Iterambere ryacu riterwa nibicuruzwa bisumba byose, impano zikomeye kandi imbaraga zikoranabuhanga zikomezaUbushinwa PCR Kwiyongera na Qpcr, Isosiyete yacu ikora ikurikiza ihame ryibikorwa by "ubunyangamugayo bushingiye, ubufatanye bwashyizweho, abantu berekeza, ubufatanye-bunguka".Turizera ko dushobora kugirana ubucuti numucuruzi uturutse impande zose zisi.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice I.	Buffer P1
	Buffer P2
	6 Buffer Yipakurura ADN
Igice cya II	2 × Ibibabi PCR Byoroshye^TMKuvanga (UNG)

Ibiranga ibyiza

■ Ntabwo bitwara igihe kandi bihenze bya ADN
Material Ibikoresho bike
■ Byoroheje-icyitegererezo gishobora gukorerwa PCR reaction cyangwa lysis reaction itagabanije cyangwa gusya
■ 2 × Kuvanga, kugabanya icyitegererezo cyo gupakira hamwe nigihe cyo gutegura sisitemu yo gutegura
Preparation Imyiteguro yihuse-yuzuye irashobora kurangira muminota 10, PCR reaction irashobora kurangira byibuze muminota 50
■ Ibisubizo byinshi-lysis reaction irashobora kurangizwa muri plaque 96-nziza ya PCR

Ibipimo by'ibikoresho

Gusaba: kumenyekanisha genetike, kumenyekanisha genotyping, nibindi.
Icyitegererezo: ikibabi cyibiti
Igipimo: Diameter 2-3mm (uburyo butaziguye), diameter 5-7mm (uburyo bwa lysis)
Urwego rwo gutahura: igice cyintego ≤1kb

Urujya n'uruza rw'akazi

Gutera ibibabi bitaziguye PCR Kit-UNG4

Amashanyarazi

Gutera ibibabi bitaziguye PCR Kit-UNG5

Ibyumweru bibiri bya PCR ivanga ibisubizo bivuye muburyo bwo kwanduza ibicuruzwa bya PCR.Iterambere ryacu riterwa nibicuruzwa bisumba byose, impano zikomeye kandi byongerewe imbaraga imbaraga zikoranabuhanga kubashinwa babigize umwuga Bikora cyane kandi bihamye Long Taq DNA Polymerase hamwe na Loading Buffer hamwe na DNTP PCR Kit, Hamwe nubuyobozi bwinganda, ubucuruzi bwagiye bwiyemeza gushyigikira ibyifuzo byubu kugirango babe umuyobozi wamasoko mubikorwa byabo.
Abashinwa b'umwugaUbushinwa PCR Kwiyongera na Qpcr, Isosiyete yacu ikora ikurikiza ihame ryibikorwa by "ubunyangamugayo bushingiye, ubufatanye bwashyizweho, abantu berekeza, ubufatanye-bunguka".Turizera ko dushobora kugirana ubucuti numucuruzi uturutse impande zose zisi.

Mbere: PCR reagents kugirango ikoreshwe cyane ya molekuline ya biologiya ikoreshwa mubushakashatsi

Ibikurikira: Nucleic Acide yoza ibikoresho byo gukuramo ibikoresho Rna kwigunga

Andika ubutumwa bwawe hano hanyuma utwohereze