Ubushinwa nucleic aside ikuramo ibikoresho Gukora nuwabitanga |Foregene

ibikoresho byo gukuramo aside nucleic

nucleic aside ikuramo ibikoresho Byerekanwe Ishusho

Ibisobanuro:

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe;Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Sisitemu yose ni RNase-Yubusa, kugirango RNA isukuye ntisuzugurwe;Buffer RW1, Buffer RW2 sisitemu yo gukaraba ya garanti ya RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda w’ibinyabuzima.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Twiyemeje gutanga byoroshye, bizigama igihe kandi bizigama amafaranga yo kugura inkunga imwe yo kugura abaguzi kubikoresho byo gukuramo aside nucleic, Twishimiye byimazeyo abaguzi bo mumahanga kwerekeza kubufatanye bwigihe kirekire hiyongereyeho iterambere.
Twiyemeje gutanga byoroshye, kuzigama igihe no kuzigama amafaranga imwe yo kugura inkunga kubaguzi kuriIkizamini cya Acide Nucleic, Mubyukuri, igiciro cyo gupiganwa, igipapuro gikwiye hamwe nogutanga mugihe bizizezwa nkuko abakiriya babisabwa.Turizera byimazeyo kubaka umubano wubucuruzi nawe dushingiye ku nyungu ninyungu mugihe cya vuba.Murakaza neza cyane kutwandikira no kutubera abafatanyabikorwa.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Ibigize ibikoresho

Buffer cRL1, Buffer cRL2

Buffer RW1, Buffer RW2

Ubusa ddH₂O, Inkingi-yoza

RNA-Inkingi gusa

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

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Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

selile yose RNA

Igishushanyo

Akagari Igiteranyo Cyuzuye RNA Igikoresho Cyakazi Flow1

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka).

Twiyemeje gutanga byoroshye, bizigama igihe kandi bizigama amafaranga imwe yo kugura abaguzi kuri OEM / ODM Ubushinwa Ubushinwa Nokonden Mobile ThermometricIkizamini cya Acide NucleicBooth, Twishimiye byimazeyo abaguzi bo mumahanga kwerekeza kubufatanye burambye hiyongereyeho iterambere.
OEM / ODM Ubushinwa Ubushinwa Ubushyuhe,Ikizamini cya Acide Nucleic, Mubyukuri, igiciro cyo gupiganwa, igipapuro gikwiye hamwe nogutanga mugihe bizizezwa nkuko abakiriya babisabwa.Turizera byimazeyo kubaka umubano wubucuruzi nawe dushingiye ku nyungu ninyungu mugihe cya vuba.Murakaza neza cyane kutwandikira no kutubera abafatanyabikorwa.

Mbere: SARS-CoV-2 Ibintu bitandukanye bya Nucleic Acide Detection Kit III (Multiplex PCR Fluorescent Probe Method) -kumenya mutant wa Delta

Ibikurikira: Igiciro Cyinshi Ubushinwa Ctni ICU Ivd Poct Byihuta Ikizamini Ikarita Yumutima Myocardium Marker

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