Ubushinwa Bwiza Bwiza Mubushinwa PCR Icyitegererezo Gutegura Automatic Nucleic Acide Isukura Amashanyarazi nuwitanga |Foregene

Ubwiza buhanitse kubushinwa PCR Icyitegererezo Gutegura Automatic Nucleic Acide Isukura

Ubwiza Bwiza Kubushinwa PCR Icyitegererezo Gutegura Automatic Nucleic Acide Isukura Ikuramo Ishusho

Ibisobanuro:

Iki gikoresho gikoresha ikoranabuhanga rya Real-RT RT (rRT-PCR) kugirango hamenyekane neza SARS-CoV-2 (ORF1ab gene na N gene) nucleic acide muri nasofaryngeal cyangwa oropharyngeal swab sample, hakoreshejwe uburyo bwa multiplex PCR fluorescent probe.Iki gikoresho kirimo igenzura ryimbere kugirango risuzume ubwiza bwikigereranyo, bityo ntigikenewe kwipimisha nuclée nini,

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Kubona ibyifuzo byabaguzi nintego yisosiyete yacu itagira iherezo.Tuzakora ingamba ziteye ubwoba kugirango tubone ibisubizo bishya kandi byujuje ubuziranenge, duhuze nibisobanuro byihariye kandi tunaguha ibicuruzwa mbere yo kugurisha, kugurisha no kugurisha nyuma yo kugurisha ibicuruzwa byiza byo mu Bushinwa PCR Icyitegererezo Gutegura Automatic Nucleic Acide Purification Extractor, serivisi nziza, mugihe gikwiye hamwe nigiciro cyo kugurisha Agressive, byose bitugira izina ryiza murwego rwa xxx.
Kubona ibyifuzo byabaguzi nintego yisosiyete yacu itagira iherezo.Tuzakora ibikorwa biteye ubwoba kugirango tubone ibisubizo bishya kandi byujuje ubuziranenge, duhuze nibisobanuro byihariye kandi tuguhe mbere yo kugurisha, kugurisha no kugurisha nyuma yo kugurishaUbushinwa Infrared Thermometero, Igikoresho cyubuzima, ubu dufite uburambe bwimyaka 8 yumusaruro nuburambe bwimyaka 5 mubucuruzi nabakiriya kwisi yose.abakiriya bacu bakwirakwijwe cyane muri Amerika ya ruguru, Afurika n'Uburayi bw'Uburasirazuba.turashobora gutanga ibicuruzwa byiza cyane hamwe nigiciro cyapiganwa cyane.

Ibisobanuro

48 Rxns, 96 Rxns

Oya.	Ibigize	Umubare				Ibice nyamukuru
Oya.	Ibigize	48 Rxns		96 Rxns		Ibice nyamukuru
1	Nucleic aside irekura	1.4mL / tube	Imiyoboro 2	5.3 mL / icupa	Icupa 1	Surfactant
2	Kurinda RNA	27 μL / umuyoboro	1 tube	53 μL / umuyoboro	1 tube	RNase inhibitor
3	SARS-CoV-2 igisubizo cyibisubizo	800 μL / umuyoboro	1 tube	1600 μL / umuyoboro	1 tube	Primer, probe, reaction reaction, dNTP
4	SARS-CoV-2 enzyme ivanze	80 μL / umuyoboro	1 tube	160 μL / umuyoboro	1 tube	Gutangira bishyushye Taq enzyme, M-MLV enzyme
5	SARS-CoV-2 kugenzura neza	100 μL / umuyoboro	1 tube	100 μL / umuyoboro	1 tube	Recombinant plasmid ikubiyemo igice cyagenewe, RNA
6	SARS-CoV-2 kugenzura nabi	1200 μL / umuyoboro	1 tube	1200 μL / umuyoboro	1 tube	TE buffer

Ibiranga ibyiza

Byihuse kandi byoroshye.
Irinde gutakaza aside nucleic.
Umupaka muto wo gutahura no kwiyumvamo byinshi.
Ibikoresho bike bisabwa no gukoresha byoroshye.

Kumenya neza birashobora kugera kurwego rwo hasi nka 500 kopi / mL).

GCE / mL	Ct agaciro
GCE / mL	N gen	ORF1ab
2.00E + 08	16.43	15.16
2.00E + 07	19.48	18.58
2.00E + 06	22.71	21.86
2.00E + 05	25.95	25.33
2.00E + 04	29.30	28.49
2.00E + 03	32.37	31.37
2.00E + 02	35.32	34.47
2.00E + 01	38.29	38.27
2.00E + 00	N / A.	N / A.
2.00E-01	N / A.	N / A.
NC	N / A.	N / A.

Umurongo wo kwongera umurongo

amplification curve-RT-qPCR

Uburyo bwo gukoresha

1. Igikoresho gikoreshwa

covid-19 nucleic aside itahura kit1

2. Uburyo bwo gutahura

covid-19 nucleic aside itahura kit2

Gutunganya icyitegererezo: Naopharyngeal cyangwa oropharyngeal swab sample (gukora intambwe 1 gusa) (Tegereza iminota 10)

PCR amplificaton (<= 55min)

Urupapuro rw'akazi:

covid-19 nucleic aside itahura kit4

Ububiko

bifunze ku mucyo kandi bibitswe kuri -20 ± 5 ℃;

ububiko
ububiko2

Ubuzima bwa Shelf:Umwaka 1

Kubona ibyifuzo byabaguzi nintego yisosiyete yacu itagira iherezo.Tuzakora ingamba ziteye ubwoba kugirango tubone ibisubizo bishya kandi byujuje ubuziranenge, duhuze nibisobanuro byihariye kandi tunaguha ibicuruzwa mbere yo kugurisha, kugurisha no kugurisha nyuma yo kugurisha ibicuruzwa byiza byo mu Bushinwa PCR Icyitegererezo Gutegura Automatic Nucleic Acide Purification Extractor, serivisi nziza, mugihe gikwiye hamwe nigiciro cyo kugurisha Agressive, byose bitugira izina ryiza murwego rwa xxx.
Ubuziranenge bwo hejuruUbushinwa Infrared Thermometero, Igikoresho cyubuzima, ubu dufite uburambe bwimyaka 8 yumusaruro nuburambe bwimyaka 5 mubucuruzi nabakiriya kwisi yose.abakiriya bacu bakwirakwijwe cyane muri Amerika ya ruguru, Afurika n'Uburayi bw'Uburasirazuba.turashobora gutanga ibicuruzwa byiza cyane hamwe nigiciro cyapiganwa cyane.

Mbere: Tanga Ubushinwa Nucleic Acide Ikizamini / Igikoresho cyo Kumenya Rna (Ikibazo Cyinshi cya RT-PCR Fluorescence Ikibazo (Lyophilised)

Ibikurikira: Uruganda Ubushinwa Techstar Kurandura Ibikoresho Byerekana Icyitegererezo Kurekura Reagent Ntibikenewe Rna / Gukuramo Acide Nucleic Acide Kit Reagent Kits kumwanya nyawo

Andika ubutumwa bwawe hano hanyuma utwohereze