Buri gihe dukomera ku ihame “Ubwiza Bwambere, Icyubahiro Cyikirenga”.Twiyemeje rwose guha abakiriya bacu ibicuruzwa byiza byapiganwa ku isoko, gutanga vuba na serivisi zumwuga kubisanzwe bisanzweUbushinwa Gukuramo Acide Nucleic Reagent.Ibicuruzwa byacu ufite agaciro.
Buri gihe dukomera ku ihame “Ubwiza Bwambere, Icyubahiro Cyikirenga”.Twiyemeje rwose guha abakiriya bacu ibicuruzwa byiza byapiganwa ku isoko, gutanga vuba na serivisi zumwuga kuriUbushinwa Gukuramo Acide Nucleic Reagent, Uburyo bwa Magnetique, Politiki y'Ikigo cyacu "ubuziranenge ubanza, kugirango turusheho kuba bwiza no gukomera, iterambere rirambye".Intego zacu zo gukurikirana ni "societe, abakiriya, abakozi, abafatanyabikorwa ninganda gushaka inyungu zifatika".Turashaka gukora ubufatanye nabakora ibice bitandukanye byimodoka, gusana amaduka, urungano rwimodoka, hanyuma turema ejo hazaza heza!Urakoze gufata umwanya wo kureba kurubuga rwacu kandi twakwemera icyifuzo icyo ari cyo cyose waba ufite cyadufasha kunoza urubuga.

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Buri gihe dukomera ku ihame “Ubwiza Bwambere, Icyubahiro Cyikirenga”.Twiyemeje guha abakiriya bacu ibicuruzwa byiza byapiganwa ku isoko, gutanga vuba na serivisi zumwuga kubisanzwe bigabanywa Ubushinwa Nucleic Acide Extraction Reagent (Serum / Plasma na Puncture solution), Rna Extraction Test Kits, Twibanze ku kubaka ikirango cyacu kandi duhujwe n’imvugo myinshi inararibonye hamwe nibikoresho byo mu rwego rwa mbere.Ibicuruzwa byacu ufite agaciro.
Kugabanuka bisanzwe Ubushinwa Gukuramo Acide Nucleic Reagent,Uburyo bwa Magnetique, Politiki y'Ikigo cyacu "ubuziranenge ubanza, kugirango turusheho kuba bwiza no gukomera, iterambere rirambye".Intego zacu zo gukurikirana ni "societe, abakiriya, abakozi, abafatanyabikorwa ninganda gushaka inyungu zifatika".Turashaka gukora ubufatanye nabakora ibice bitandukanye byimodoka, gusana amaduka, urungano rwimodoka, hanyuma turema ejo hazaza heza!Urakoze gufata umwanya wo kureba kurubuga rwacu kandi twakwemera icyifuzo icyo ari cyo cyose waba ufite cyadufasha kunoza urubuga.