SARS-CoV-2 Ibintu bitandukanye bya Nucleic Acide Detection Kit III (Multiplex PCR Fluorescent Probe Method) -kumenya mutant wa Delta
Ibisobanuro:
PCR itaziguye:Ntibikenewe ko acide nucleic acide kit na Sisitemu, byujujwe ingero 96 mumasaha 1 cyangwa arenga.
Equipment Ibikoresho bike bisabwa no gukoresha byoroshye:Gusa ukeneye sisitemu nyayo-Igihe PCR.
◮ LoD hamwe no kumva neza:Kumenya gushishoza birashobora kugera kurwego rwo hasi nka 500 kopi / ml.
Ibisobanuro
48 Rxns / kit, 96 Rxns / kit
| Oya. | Ibigize | Umubare | Ibyingenzi | |||
| 48 Rxns | 96 Rxns | |||||
| 1 | Nucleic aside irekura | 1.4 mL / umuyoboro | Imiyoboro 2 | 5.3 mL / icupa | Icupa 1 | Surfactant |
| 2 | Kurinda RNA | 53 μL / umuyoboro | 1 tube | 106 μL / umuyoboro | 1 tube | RNase inhibitor |
| 3 | SARS-CoV-2 Ibitandukanye igisubizo A. | 800 μL / umuyoboro | 1 tube | 1600 μL / umuyoboro | 1 tube | Primer, probe, reaction reaction, dNTP |
| 4 | SARS-CoV-2 enzyme ivanze | 160 μL / umuyoboro | 1 tube | 320 μL / umuyoboro | 1 tube | Gutangira bishyushye Taq enzyme, M-MLV enzyme |
| 5 | SARS-CoV-2 kugenzura neza | 100 μL / umuyoboro | 1 tube | 100 μL / umuyoboro | 1 tube | Recombinant plasmid ikubiyemo igice cyagenewe, RNA |
| 6 | SARS-CoV-2 kugenzura nabi | 1200 μL / umuyoboro | 1 tube | 1200 μL / umuyoboro | 1 tube | TE buffer |
Ibiranga ibyiza:
Byihuse kandi byoroshye: uzuza ikizamini mugihe cyamasaha 1
Irinde gutakaza aside nucleic: ntukeneye kweza aside nucleic
Umupaka muto wo gutahura no kwiyumvamo cyane, cyane cyane suitabe kugirango nini nini yihuse
Ibikoresho bike bisabwa no gukoresha byoroshye
Kumenya neza birashobora kugera kurwego rwo hasi nka 500 kopi / mL).
Uburyo bwo gukoresha:
1.Ibikoresho bikoreshwa
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
2.Uburyo bwo kumenya
Gutunganya icyitegererezo: Naopharyngeal cyangwa oropharyngeal swab sample (intambwe 1 gusa)Tegereza iminota 10)
PCR amplificaton (<= 55min)
Urupapuro rw'akazi:
Ububiko:
Ikidodo kiva mumucyo kandi kibitswe kuri -20 ± 5 ℃;
Ubike kuri 2-8 ℃ nyuma yo gufungura, koresha muminsi 7;irinde gukonjesha inshuro nyinshi ( Ubuzima bwa Shelf:Umwaka 1
