Ubushinwa SARS-CoV-2 Nucleic Acide Detection Kit (Multiplex PCR Fluorescent Probe Method) Ihingura nuwitanga |Foregene

SARS-CoV-2 Nucleic Acide Detection Kit (Multiplex PCR Fluorescent Probe Method)

Ibisobanuro:

PCR itaziguye:Ntibikenewe ko acide nucleic acide kit na Sisitemu, byujujwe ingero 96 mumasaha 1 cyangwa arenga.
Equipment Ibikoresho bike bisabwa no gukoresha byoroshye:Gusa ukeneye sisitemu nyayo-Igihe PCR.
◮ LoD hamwe no kumva neza:Kumenya gushishoza birashobora kugera kurwego rwo hasi nka 500 kopi / ml.

Ohereza imeri kuri twe Kuramo nka PDF

Ibicuruzwa birambuye

Ibicuruzwa

Gukoresha

Iki gikoresho gikoresha tekinoroji ya RT PCR (rRT-PCR) kugirango hamenyekane neza acide nucleic acide ya SARS-CoV-2 muri acide ya nasofaryngeal cyangwa oropharyngeal swab.

Ihame ryo kwipimisha

Iyi primer na probe byashizweho kugirango hamenyekane urutonde rwabitswe rwa SARS-CoV-2 (gene ya ORF1ab, N gene na E gene).Igikoresho kirimo Igenzura ryimbere rikoreshwa mugusuzuma ubuziranenge bwikigereranyo.Ikoranabuhanga nyaryo PCR ikoresha polymerase urunigi (PCR) kugirango hongerwe intego yihariye hamwe nubushakashatsi bwihariye bwo kumenya RNA yongerewe.Iperereza ryanditseho umunyamakuru wa fluorescent hamwe namabara yo kuzimya.

Ibisobanuro Byihuse

◮Ibisobanuro:48 Rxns / kit, 96 Rxns / kit

Umwihariko:96,72%

Ibyiyumvo:97,92%

Ubwoko bwikigereranyo bwakoreshwa

Nasopharyngeal Swab cyangwa Oropharyngeal Swab

◮Igikoresho gikoreshwa

ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S

Ibigize

Oya.	Ibigize	Umubare				Ibyingenzi
Oya.	Ibigize	48 Rxns		96 Rxns		Ibyingenzi
1	Kurekura aside nucleiqueumukozi	1.5 mL / umuyoboro	1 tube	1.5 mL / umuyoboro	1 tube	Surfactant
2	Kurinda RNA	27 μL / umuyoboro	1 tube	53 μL / umuyoboro	1 tube	RNase inhibitor
3	SARS-CoV-2igisubizo	800 μL / umuyoboro	1 tube	1600 μL / umuyoboro	1 tube	Primer, probe, reaction reaction, dNTP
4	SARS-CoV-2enzyme ivanze	80 μL / umuyoboro	1 tube	160 μL / umuyoboro	1 tube	Gutangira bishyushye Taq enzyme,M-MLV enzyme
5	SARS-CoV-2kugenzura neza	100 μL / umuyoboro	1 tube	100 μL / umuyoboro	1 tube	Recombinant plasmid irimo integoagace, RNA
6	SARS-CoV-2kugenzura nabi	1200 μL / umuyoboro	1 tube	1200 μL / umuyoboro	1 tube	TE buffer