Buri gihe twizera ko imiterere yumuntu igena ubuziranenge bwibicuruzwa, ibisobanuro birambuye bigena ubuziranenge bwibicuruzwa, hamwe numwuka wikipe ya REALISTIC, EFFICIENT NA INNOVATIVE kubushinwa Ibiciro-Bikora-Inganda zikora ibicuruzwa byinshi ADN Rna Isolation Kit Reagents, Twakiriye abaguzi, amashyirahamwe yubucuruzi hamwe nabashakanye baturutse mubice byose kwisi kugirango batubwire kandi dushake ubufatanye muburyo bwiza.
Twama twizera ko imico yumuntu igena ubuziranenge bwibicuruzwa, ibisobanuro birambuye bigena ubuziranenge bwibicuruzwa, hamwe numwuka wikipe NYAKURI, INGARUKA NA INNOVATIVE kuriUbushinwa Universal Rna ADN Reagents, Kubera ibicuruzwa byiza na serivisi byacu, ubu twabonye izina ryiza kandi ryizewe kubakiriya baho ndetse n’amahanga.Niba ukeneye amakuru menshi kandi ushishikajwe nikintu icyo aricyo cyose, menya neza ko utwiyambaza.Dutegereje kuzaba abaguzi bawe mugihe cya vuba.

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Buri gihe twizera ko imiterere yumuntu igena ubuziranenge bwibicuruzwa, ibisobanuro birambuye bigena ubuziranenge bwibicuruzwa, hamwe numwuka witsinda NYAKURI, RIKORESHEJWE NA INNOVATIVE kumpamvu yo gutoranya cyane kubushinwa Ibiciro-Bikora-Inganda zikora ibicuruzwa byinshi ADN Rna Isolation Kit Reagents, Twakiriye abaguzi, amashyirahamwe yubucuruzi hamwe nabashakanye mubice byose kwisi kugirango tubone amakuru meza kandi dushake ubufatanye.
Guhitamo Byinshi KuriUbushinwa Universal Rna ADN Reagents, Automatic Nucleic Acide Isukura, Kubera ibicuruzwa na serivisi nziza, ubu twabonye izina ryiza kandi ryizewe kubakiriya baho ndetse n’amahanga.Niba ukeneye amakuru menshi kandi ushishikajwe nikintu icyo aricyo cyose, menya neza ko utwiyambaza.Dutegereje kuzaba abaguzi bawe mugihe cya vuba.