Hamwe niyi ntego, twahinduye umwe mubishoboka cyane guhanga udushya mu ikoranabuhanga, gukoresha amafaranga menshi, no guhatanira ibiciro kubiranga ubuziranenge.Ubushinwa Gukuramo Acide Nucleic Reagent, Rna Gukuramo Ikizamini Kits Nucleic Acide Gukuramo Reagent, Murakaza neza rwose ko mutubera uruhande rumwe kugirango tworohereze umuryango wawe.Mubisanzwe twabaye umufatanyabikorwa wawe ukomeye mugihe ushaka kugira umushinga wawe muto.
Hamwe niyi ntego, twahinduye umwe mubishoboka cyane guhanga udushya mu ikoranabuhanga, gukoresha amafaranga menshi, no guhatanira ibiciro kuriUbushinwa Gukuramo Acide Nucleic Reagent, Uburyo bwa Magnetique, Tugiye gukora ibishoboka byose kugirango dufatanye & banyuzwe nawe twishingikirije ku rwego rwo hejuru rwo hejuru no ku giciro cyo gupiganwa kandi byiza nyuma ya serivisi, dutegereje tubikuye ku mutima gufatanya nawe no kugera ku bikorwa mu gihe kizaza!

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.

Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe.

Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Ibigize ibikoresho

* Nyamuneka wambare uturindantoki kandi ufate ingamba zo kubarinda mugihe cyo kubaga kuko Buffer cRL1 na Buffer RW1 zirimo umunyu wa chaotropique.

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

Igishushanyo

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka). Hamwe niyi nteruro mubitekerezo, twahinduye umwe mubashoboka ko bakora udushya twikoranabuhanga, dukoresha amafaranga menshi, kandi duhatanira igiciro cyiza cyane.Ubushinwa Gukuramo Acide Nucleic Reagent, Rna Gukuramo Ikizamini Kits Nucleic Acide Gukuramo Reagent, Murakaza neza rwose ko mutubera uruhande rumwe kugirango tworohereze umuryango wawe.Mubisanzwe twabaye umufatanyabikorwa wawe ukomeye mugihe ushaka kugira umushinga wawe muto.
Ubuziranenge Bwiza Ubushinwa Gukuramo Acide Nucleic Reagent,Uburyo bwa Magnetique, Tugiye gukora ibishoboka byose kugirango dufatanye & banyuzwe nawe twishingikirije ku rwego rwo hejuru rwo hejuru no ku giciro cyo gupiganwa kandi byiza nyuma ya serivisi, dutegereje tubikuye ku mutima gufatanya nawe no kugera ku bikorwa mu gihe kizaza!