"Ubwiza bwambere, Kuba inyangamugayo nkibishingiro, ubufasha buvuye ku mutima no kunguka inyungu" nigitekerezo cyacu, nkuburyo bwo gutanga umusaruro uhoraho no gukurikirana indashyikirwa kubiciro byinshi byo kugurisha Ubushinwa Igiciro-Cyiza Cyakozwe ninganda nyinshi ADN Rna Isolation Kit Reagents, Biturutse kumasoko yubaka byihuse yibiribwa byihuse nibinyobwa bikoreshwa kwisi yose, Turashaka ko dukorana nabafatanyabikorwa / abakiriya.
"Ubwiza bwambere, Kuba inyangamugayo nkibishingiro, ubufasha buvuye ku mutima no kunguka inyungu" nigitekerezo cyacu, nkuburyo bwo gutanga umusaruro uhoraho no gukurikirana ibyiza kuriAutomatic Nucleic Acide, Ubushinwa Universal Rna ADN Reagents, Isosiyete yacu ubu ifite amashami menshi, kandi ifite abakozi barenga 20 muri sosiyete yacu.Twashizeho iduka ryo kugurisha, icyumba cyo kwerekana, hamwe nububiko bwibicuruzwa.Hagati aho, twanditse ikirango cyacu.Twakomeje kugenzura ubuziranenge bwibicuruzwa.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.

Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe.

Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Ibigize ibikoresho

* Nyamuneka wambare uturindantoki kandi ufate ingamba zo kubarinda mugihe cyo kubaga kuko Buffer cRL1 na Buffer RW1 zirimo umunyu wa chaotropique.

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

Igishushanyo

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (itabishaka). , Turashaka imbere gukorana nabafatanyabikorwa / abakiriya kugirango dukore ibisubizo byiza hamwe.
Igiciro CyinshiUbushinwa Universal Rna ADN Reagents, Automatic Nucleic Acide, Isosiyete yacu ubu ifite amashami menshi, kandi ifite abakozi barenga 20 muri sosiyete yacu.Twashizeho iduka ryo kugurisha, icyumba cyo kwerekana, hamwe nububiko bwibicuruzwa.Hagati aho, twanditse ikirango cyacu.Twakomeje kugenzura ubuziranenge bwibicuruzwa.