Intego yacu hamwe nishirahamwe ni "Guhora twuzuza ibyo abakiriya bacu bakeneye".Turakomeza guteza imbere no gutunganya ibintu bitangaje byujuje ubuziranenge kuri buri muguzi wacu ushaje kandi mushya kandi tugakora ibyifuzo byunguka kubakiriya bacu kimwe natwe ku ruganda KubushinwaAcide Nucleic Acide& Kwoza ibikoresho (MagPure Universal RNA Kit), Ihame ryibikorwa byacu nukugaragaza ibintu byujuje ubuziranenge, inkunga yinzobere, hamwe n’itumanaho ryukuri.Ikaze inshuti zose za hafi kugirango ugure ikigeragezo cyo guteza imbere umubano wigihe kirekire.
Intego yacu hamwe nishirahamwe ni "Guhora twuzuza ibyo abakiriya bacu bakeneye".Turakomeza guteza imbere no gutunganya ibintu bitangaje byujuje ubuziranenge kuri buri muguzi wacu ushaje kandi mushya kandi tugakora ibyiringiro-byunguka kubakiriya bacu nkatweUbushinwa Rna / Gukuramo ADN, Acide Nucleic Acide, Igitekerezo cyacu ni "ubunyangamugayo mbere, ubuziranenge bwiza".Dufite ibyiringiro byo kuguha serivisi nziza nibicuruzwa byiza.Turizera tubikuye ku mutima ko dushobora gushyiraho ubufatanye mu bucuruzi-bunguka nawe ejo hazaza!

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.

Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe.

Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Ibigize ibikoresho

* Nyamuneka wambare uturindantoki kandi ufate ingamba zo kubarinda mugihe cyo kubaga kuko Buffer cRL1 na Buffer RW1 zirimo umunyu wa chaotropique.

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

Igishushanyo

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka) .Icyifuzo cyacu hamwe nishirahamwe ni "Guhora twujuje ibyifuzo byabakiriya bacu".Turakomeza guteza imbere no gutunganya ibintu bitangaje byujuje ubuziranenge kuri buri muguzi wacu ushaje kandi mushya kandi tugakora ibyifuzo byunguka kubakiriya bacu kimwe natwe ku ruganda KubushinwaAcide Nucleic Acide& Kwoza ibikoresho (MagPure Universal RNA Kit), Ihame ryibikorwa byacu nukugaragaza ibintu byujuje ubuziranenge, inkunga yinzobere, hamwe n’itumanaho ryukuri.Ikaze inshuti zose za hafi kugirango ugure ikigeragezo cyo guteza imbere umubano wigihe kirekire.
Uruganda KuriUbushinwa Rna / Gukuramo ADN, Nucleic Acide Isolation, Igitekerezo cyacu ni "ubunyangamugayo mbere, ubuziranenge bwiza".Dufite ibyiringiro byo kuguha serivisi nziza nibicuruzwa byiza.Turizera tubikuye ku mutima ko dushobora gushyiraho ubufatanye mu bucuruzi-bunguka nawe ejo hazaza!