Ishirahamwe ryacu ryubahirije ihame ryawe rya "Ubwiza bushobora kuba ubuzima bwumuryango wawe, kandi izina rizaba roho yacyo" "Uruganda rwatanze Ubushinwa Bwabigize umwuga Ubuvuzi PCR Ikizamini Igihe nyacyo PCR Imashini igurishwa, Hamwe nibintu byinshi, byujuje ubuziranenge, amafaranga yemewe hamwe nubufasha bukomeye, tugiye kuba umufatanyabikorwa wawe mwiza wubucuruzi.Twishimiye ibyifuzo bishya kandi byabanjirije ibyiciro byose byubuzima kugirango tubonane natwe kubucuruzi buciriritse bwimishinga no kugera kubyo twageraho!
Ishirahamwe ryacu rikurikiza amahame yawe ya "Ubwiza bushobora kuba ubuzima bwumuryango wawe, kandi icyubahiro kizaba ubugingo bwacyo" kuriGutahura Acide Nucleic Acide, Ubushinwa Rt PCR Ibikoresho, Perezida hamwe n’abanyamuryango bose b’isosiyete barashaka gutanga ibicuruzwa na serivisi byinzobere kubakiriya kandi bakira byimazeyo kandi bagafatanya nabakiriya bose kavukire ndetse nabanyamahanga kugirango ejo hazaza heza.

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Ishirahamwe ryacu ryubahirije ihame ryawe rya "Ubwiza bushobora kuba ubuzima bwumuryango wawe, kandi izina rizaba roho yacyo" "Uruganda rwatanze Ubushinwa Bwabigize umwuga Ubuvuzi PCR Ikizamini Igihe nyacyo PCR Imashini igurishwa, Hamwe nibintu byinshi, byujuje ubuziranenge, amafaranga yemewe hamwe nubufasha bukomeye, tugiye kuba umufatanyabikorwa wawe mwiza wubucuruzi.Twishimiye ibyifuzo bishya kandi byabanjirije ibyiciro byose byubuzima kugirango tubonane natwe kubucuruzi buciriritse bwimishinga no kugera kubyo twageraho!
Uruganda rwatanzweUbushinwa Rt PCR Ibikoresho, Gutahura Acide Nucleic Acide, Perezida hamwe n’abanyamuryango bose b’isosiyete barashaka gutanga ibicuruzwa na serivisi byinzobere kubakiriya kandi bakira byimazeyo kandi bagafatanya nabakiriya bose kavukire ndetse nabanyamahanga kugirango ejo hazaza heza.