Buri gihe dutekereza kandi tugakora imyitozo ijyanye no guhindura ibihe, tugakura.Dufite intego yo kugera ku bwenge n'umubiri bikungahaye kimwe no kubaho kubushakashatsi bwihariye kubushakashatsi bwa kaminuza yo mu Bushinwa Koresha Impapuro PCR Downstream Experiment Automatic Nucleic Acide Extractor, Gukomeza kuboneka ibicuruzwa byinshi byo mu rwego rwo hejuru hamwe ninkunga idasanzwe yabanjirije na nyuma yo kugurisha bituma irushanwa rikomeye ku isoko ryiyongera ku isi.
Buri gihe dutekereza kandi tugakora imyitozo ijyanye no guhindura ibihe, tugakura.Dufite intego yo kugera kumitekerereze ikungahaye kumubiri no mubuzima kimwe no kubahoAutomatic Nucleic Acide ikuramo, Ubushinwa Automatic Nucleic Acide Isukura, Isosiyete yacu yubatse umubano uhamye wubucuruzi hamwe namasosiyete menshi azwi yo murugo ndetse nabakiriya bo hanze.Dufite intego yo gutanga ibicuruzwa byujuje ubuziranenge nibisubizo kubakiriya kuri cote nkeya, twiyemeje kuzamura ubushobozi bwayo mubushakashatsi, iterambere, gukora no gucunga.Twishimiye kwakira abakiriya bacu.Kugeza ubu twatsinze ISO9001 muri 2005 na ISO / TS16949 muri 2008. Ibigo by "ubuzima bwiza, kwizerwa kwiterambere" kubwintego, byakira byimazeyo abacuruzi bo mu gihugu ndetse n’abanyamahanga gusura kugira ngo baganire ku bufatanye.

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Buri gihe dutekereza kandi tugakora imyitozo ijyanye no guhindura ibihe, tugakura.Dufite intego yo kugera ku bwenge n'umubiri bikungahaye kimwe no kubaho kubushakashatsi bwihariye kubushakashatsi bwa kaminuza yo mu Bushinwa Koresha Impapuro PCR Downstream Experiment Automatic Nucleic Acide Extractor, Gukomeza kuboneka ibicuruzwa byinshi byo mu rwego rwo hejuru hamwe ninkunga idasanzwe yabanjirije na nyuma yo kugurisha bituma irushanwa rikomeye ku isoko ryiyongera ku isi.
Igishushanyo cyihariye cyaUbushinwa Automatic Nucleic Acide Isukura, Automatic Nucleic Acide ikuramo, Isosiyete yacu yubatse umubano uhamye wubucuruzi hamwe namasosiyete menshi azwi yo murugo ndetse nabakiriya bo hanze.Dufite intego yo gutanga ibicuruzwa byujuje ubuziranenge nibisubizo kubakiriya kuri cote nkeya, twiyemeje kuzamura ubushobozi bwayo mubushakashatsi, iterambere, gukora no gucunga.Twishimiye kwakira abakiriya bacu.Kugeza ubu twatsinze ISO9001 muri 2005 na ISO / TS16949 muri 2008. Ibigo by "ubuzima bwiza, kwizerwa kwiterambere" kubwintego, byakira byimazeyo abacuruzi bo mu gihugu ndetse n’abanyamahanga gusura kugira ngo baganire ku bufatanye.